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Showing: 281 - 300 of 571 results

  1. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...insect cells require demanding culture conditions similar to mammalian expression systems. Bacterial expression...expression. Protein expression in bacteria is quite simple; DNA coding for your protein of interest is inserted...

  2. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...End point PCR, which is commonly referred to as simply PCR, is used for an array of different molecular... or pathogen identification. Scientists can simultaneously detect several different pathogens in one specimen...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction pathways... a reaction pathway, the MPyV system provides a simple, efficient method for protecting a single enzyme...

  5. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...after transforming them into your cells, you can simply look for blue colonies to determine which cells...cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using these...

  6. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...transfer plasmid. The good news is there’s a few simple ways to deal with this issue. One way is to grow...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials...detailed below. Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to...

  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ... CRISPR/Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR...

  9. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...Pooled lentiviral CRISPR libraries (referred to simply as “CRISPR libraries”) consist of a heterogeneous...

  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully engineered...incredible hidden story here. The parts we are releasing simply plug into this existing economic activity. People...

  12. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...

  13. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...

  14. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...

  15. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...

  17. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...non-scientist friends and family is Part Five, called simply “Bad Science.” Zimmer kicks off the section saying...provides several. In Part Three of the book, called simply “Old Science,” he describes the now infamous tale...

  18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...

  19. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...doors. It would be a nightmare if we didn’t have a simple way to track all of our 40,000 samples available...  If a plate of samples is ever dropped, we can simply scan the tubes and know what is in each one. Even...
Showing: 281 - 300 of 571 results