We narrowed to 309 results for: Scrib

...combining the AAV and CRISPR-based approaches is described in several recent reviews (Howes and Schofield...

...plasma membrane. In previous publications, they described tools to control formation of lamellipodia and...

...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...

...C-terminus of the fluorescent protein. I prefer to describe the different parts of the fusion from N- to C-terminus...

...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...

...a new preprint, Jared Toettcher and colleagues describe pYtags, a modular system for monitoring specific...

... through Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits...

...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...

...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...

... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...

...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...

...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin...

...between the targets. Or, it might be sufficient to describe or classify the labeled structures visually; for...

...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...

... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...

...BAC DNA can be modified with any of the below described approaches.    Bacterial strain with integrated...

... on a reverse transcriptase enzyme to reverse transcribe back into DNA before being inserted into new ...

...the opsin into the correct orientation to be transcribed (Figure 2) (Sharma and Zhu, 2014). How does FLEx...