We narrowed to 353 results for: Sele

1. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...typically with an antibody. This can artificially select against PPIs that aren't stable outside the native...

2. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...even apply forces (Gayrard and Borghi 2016) to a selected protein. Overcoming current limitations to permit...

3. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   Published

   Oct. 22, 2019, 12:20 p.m.

   ...poster to the bottom. Depending on the color you select for the background, you’ll want to make sure that...

4. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...for introducing point mutations with unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et...

5. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ..., Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory synapses ...

6. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ... 2).      Table 2: Secondary antibody selections for a four-antibody panel, staining in parallel...

7. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...infection (MOI) to ensure only one copy integrates, selecting by antibiotic-resistance or single cell sorting...

8. Illuminating Epigenetics with A FRET Based Biosensor

   Type

   Blog Post

   Published

   Nov. 19, 2015, 3:30 p.m.

   ...methyllysine binding domain (chromodomain), which binds selectively to lysine-methylated peptides. This construct...

9. Plasmids 101: Degron Tags

   Type

   Blog Post

   Published

   March 14, 2024, 1:15 p.m.

   ...protein of interest, it can be degraded by this selective degrader. The novel degrader, dTAG13, is a small...

10. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...and tsPurple chromoproteins to create negative selection markers for allelic exchange. These vectors contain...

11. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...started, too much time for this project was spent selecting guides, designing primers, and running analyses...

12. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ... resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies 101: Secondary AntibodiesAntibodies...

13. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...challenge of attempting to simultaneously and selectively induce both cutting and regulation of expression...

14. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ... Plasmids and Resources page, investigators may select a pathway and identify any genes within that pathway...

15. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    ... Addgene. Addgene performs Sanger sequencing on select regions of all distributed plasmids as part of ...

16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...and the PITCh- and locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify...

17. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    Published

    July 16, 2015, 2:30 p.m.

    ...Stelzer’s group described the single-plane or selective-plane illumination microscope (SPIM). In order...

18. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ... a fitness cost and are eliminated by natural selection, gene drives distort inheritance in their favor...

19. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type

    Blog Post

    Published

    July 1, 2025, 1:15 p.m.

    ...Best Way to Elute and Store Your Plasmid DNA? Selecting Your Plasmid Purification Kit Resources on Addgene.org...

20. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...specifically cuts methylated DNA, so it will selectively destroy the template, but not newly synthesized...