...plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time to make each plasmid...others to ensure that everything will ship out in time - from assembling the boxes, to inoculating tubes...scale reagent sharing led us to calculate how much time we have saved researchers. In a recent blog post...

...Newsletter and recently visited Cambridge to spend some time learning and doing research at Novartis. Listen ...scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction - Ghanaian scientist's... opportunities in Ghana. 46:35 - end : Kwabena's time in Boston - Developing microbiome expertise. Many...Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena Duedu, PhD is ...

...serotypes may perform better (3). Brain Region  Optimal Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2...outlined in the table below. Cell Type Cortex Optimal Serotype Oligodendrocytes AAV8 > AAV2 & AAV6 ...quickly. 3. Patience Is Bitter, but Its Fruit Is Sweet Time If you require a quick onset of expression, AAV ...see that gene expression really does not take much time at all. Much of the previous literature using AAVs...3 days vs 7 days vs 14 days etc. To decrease the time until expression, it is possible to use AAV constructs...

...they've managed to keep themselves happy during their time in grad school. As you'll learn, grad school has...topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance...right that illustrate a grad student's happiness v time throughout graduate school. This is, of course, ...We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them the best...

...Though, to be fair, I think this is a conservative estimate. As a graduate student, I drank 3-4 cups of coffee...hear that we've been able to help their labs save time and resources by storing and distributing their ...about making such tools open-access. Though it is time for me to refill my large coffee cup and get back... – so if you’re running out of energy, then it’s time for a coffee run! ...

...African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement and...itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune...Zimmer’s coverage on the subject in the New York Times and on his blog, where he has offered to answer ...

...organisms and their characteristic radiance for some time – there are records describing them dating back ...back to the first century A.D. – the first time anyone truly examined what caused this glowing phenomenon...organisms (E. coli, and later C. elegans) for the first time. After Chalfie’s work, scientists really started...fluorescent proteins with new colors Around the same time, researcher Sergey Lukyanov realized that, although...

...to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence microscopes. (See left image...sample. As more than one hole is illuminated at a time, the image can be acquired more quickly. 2-Photon...single focal plane of the sample is excited at one time, limiting photobleaching. Similar to widefield, ..., the entire field of view is excited at one time and captured in a single camera exposure. This is much...technology and parallelization (7) have allowed for real-time monitoring of neuronal activity across substantial...

...systems, the individual parts are reusable, saving time and money on future assemblies. If you are new to...for eukaryotes! The technology has been used to optimize protein secretion and expression in the biotech...guides, mini screening libraries, direct edit optimization, and more. While there are many CRISPR-Cas libraries...parts can be used in a variety of organisms, saving time and money for labs with diverse applications. The...already in place. MoClo is a fantastic way to save time and money on studies that require many assemblies...

...importantly, there’s the opportunity cost of your time; an investment of months of your life that may end...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...way to do something is to do it huge, there are times when a more incremental approach is prudent. What's...      References: 1. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize...

...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we... others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly...is that if you introduce them into cells one at a time, you could identify those that confer resistance...

...and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an experiment to performing...using one of these validated gRNAs can save you the time that would be spent making and testing entirely ...CRISPR experiment.  The selection process is often time consuming and there is no guarantee that the selected...demonstrated to work in your system, it is worth spending time sequencing the genomic target to determine whether...

...skill,  time for which most researchers wouldn't have. They are motivated to get data, publish in time, defend... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...its stunning images that is has been featured in Time magazine . Other smaller competitions are now taking...

...Welcome to 2014! Get excited, it’s a new year. A time for resolutions: to wake up and get to the lab earlier...been slower to adapt, likely due to the necessary time and resource investment required to change the status...’ll be able to make updates to protocols in real time. You may also want to consider moving your paper...added or removed samples. Once you've taken some time to get organized, stop back and let us know what...

... eight times faster than GRABNE and deactivated three times faster. This enhanced response time allows...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...

... stage, before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for ...and QC assays to be completed and reviewed. The timeline therefore will be as follows:  Request review:...Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international; varies...

...account for three years and have only tweeted 6 times: #fail. I’d hoped to use Twitter professionally ...scientists tweet URLs with scientific domains <10% of the time. Figure 3: Top 20 Domains Tweeted by Scientists... contain scientific domains between 0-10% of the time; however, ~20% of life scientists  (biologists, ...ecologists) tweet scientific domains 20% or more of the time (red box). 8. Scientists tend to follow other scientists...

...took the time to answer our questions.   Advice for success in academia How can we shape our time in academia...passion. “Love what you are doing!” It helps when the time comes to work with full force. George Church 1....during their studies. They suggest that you make time for extracurricular activities both to develop new...

...with a master’s. At the time my degree felt like a consolation prize, but in time I realized its value. ...helped develop my science communication skills. Ultimately, my degree prepared me to be exactly the type...1. Are you interested in master’s-level jobs? Ultimately a degree is a means to gain employment, so it...

...in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the samples...AAVs, it typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the cells may...concentrations or AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016): 2718...