We narrowed to 830 results for: ache

...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ... great introduction to the financial world. It teaches you about selling products which is an important...

...you don’t have a formal connection, you can just reach out to start the conversation. Many researchers ...establishing collaborations, so it doesn’t hurt to reach out and ask if they would be willing to host you...available to directly help you during your visit. Reach out to your bench mentor (if you have one) to communicate...of their way to help you with your research and teach you new techniques. Show up on time (don’t make ...

...workflows are limited by costly library prep, as each sample requires an individual library. The plexWell...DNA-barcoding step that uniquely labels DNA from each well of a 96-well plate. Then, samples from several...procedure, the quantities of library molecules for each sample remain highly normalized across a wide range...individual input DNA samples are brought in line with each other before proceeding to library prep. Even with...

...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...with a valid email address).  One may also simply attach important references that serve to further characterize...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...Techniques - Which is best for me? A Practical Approach to Choosing the B(right)est Fluorescent Protein...

...while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After realizing...safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind Gram’s crystal ...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol...

...2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues that are necessary...fluorescence. When these parts are separated from each other, the protein cannot fluoresce. When β10 and...FlipGFP were redesigned so that they are parallel to each other and thus can no longer fit with β1-9. They...dynamic range and high brightness. This reporter achieves approximately 100-fold increase in fluorescence...

...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources... related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated ...fluorescence microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will...community organizations and workforce projects to reach audiences beyond our plasmid depositors and requesters...

... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

...teams of individuals who enjoy and are experts in each of the individual tasks required to develop the ... project gets left undone or is duplicated. With each component of the project completed by an individual...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...that other people in the team grow and develop to reach their full potential in the organization. This will...

...activities – did you volunteer to do science outreach and teach children about science? Or perhaps you have...employer. For example, my experience in science outreach and writing was how I got my current job as a ...curiositycollider.org/) , which she co-founded, and the Outreach Manager for Science Borealis (hyperlink http:/...

... amongst the team so getting everyone talking to each other cannot be undervalued.” Connie Cepko points...are probably because a PI does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager...message Starting a lab can be a challenging process. Each PI emphasizes the importance of slowly building ...Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia  ...

...were tested individually in 4 marmosets each. Brain potency for each individual is reported in Figure 4B ... be best served by a dual vector, split intein approach.   Q3: What’s the best method for titering AAV...backbone and so will very likely cross-react with each other. Multiple labs have tried to transfer PHP....

...for expression of the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs... functional VH and VL sequence was not known for each of the mAbs, given the investment it would take ...community with the plasmid sequences available from each plasmid webpage. Importantly, the methods developed...are also secondary antibodies that are specific to each subclass. Approximately 70% of mouse mAbs are IgG1...

... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...assays Multiplex assays involve multiple antibodies, each with a different signaling molecule, that all need... Rachel Leeson...

...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...lysogenic. Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into...bacterium to make a small “bridge” or sex pilus that attaches to the recipient cell drawing it close. Once in...

... drawbacks with these approaches that makes it challenging for users to achieve targeted diversification... by the Dueber Lab at Berkeley, provides a new approach for generating novel genetic variants in bacteria...is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth conditions in...

...all experimental approaches, knowledge of both the advantages and limitations of each Cas enzyme can help...Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which...enzymes may better suit specific experimental approaches. One example is CasX, characterized by the Oakes...

...communication specialist, Rachel, to write. Identify expertise While Rachel had antibody experience, ...they have expertise. Other times, we may need to reach out to an external expert to help us create accurate...about! You can also suggest blog topics or even reach out about writing a guest post (email [email protected]...

...originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of... If you want a plasmid for free, you can always reach out to the PI or corresponding author of the paper...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...

...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing... pregnancy, the researchers were able to examine each individual blastomere to determine its genotype....’s important to remember that the study hasn’t achieved any end goal not available with current techniques...