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We narrowed to 816 results for: ache

Showing: 281 - 300 of 816 results

  1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues that are necessary...fluorescence. When these parts are separated from each other, the protein cannot fluoresce. When β10 and...FlipGFP were redesigned so that they are parallel to each other and thus can no longer fit with β1-9. They...dynamic range and high brightness. This reporter achieves approximately 100-fold increase in fluorescence...

  2. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...workflows are limited by costly library prep, as each sample requires an individual library. The plexWell...DNA-barcoding step that uniquely labels DNA from each well of a 96-well plate. Then, samples from several...procedure, the quantities of library molecules for each sample remain highly normalized across a wide range...individual input DNA samples are brought in line with each other before proceeding to library prep. Even with...

  3. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

  4. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...teams of individuals who enjoy and are experts in each of the individual tasks required to develop the ... project gets left undone or is duplicated. With each component of the project completed by an individual...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...that other people in the team grow and develop to reach their full potential in the organization. This will...

  5. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...activities – did you volunteer to do science outreach and teach children about science? Or perhaps you have...employer. For example, my experience in science outreach and writing was how I got my current job as a ...curiositycollider.org/) , which she co-founded, and the Outreach Manager for Science Borealis (hyperlink http:/...

  6. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... amongst the team so getting everyone talking to each other cannot be undervalued.” Connie Cepko points...are probably because a PI does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager...message Starting a lab can be a challenging process. Each PI emphasizes the importance of slowly building ...Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia  ...

  7. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...all experimental approaches, knowledge of both the advantages and limitations of each Cas enzyme can help...Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which...enzymes may better suit specific experimental approaches. One example is CasX, characterized by the Oakes...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ... drawbacks with these approaches that makes it challenging for users to achieve targeted diversification... by the Dueber Lab at Berkeley, provides a new approach for generating novel genetic variants in bacteria...is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth conditions in...

  9. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...were tested individually in 4 marmosets each. Brain potency for each individual is reported in Figure 4B ... be best served by a dual vector, split intein approach.   Q3: What’s the best method for titering AAV...backbone and so will very likely cross-react with each other. Multiple labs have tried to transfer PHP....

  10. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...for expression of the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs... functional VH and VL sequence was not known for each of the mAbs, given the investment it would take ...community with the plasmid sequences available from each plasmid webpage. Importantly, the methods developed...are also secondary antibodies that are specific to each subclass. Approximately 70% of mouse mAbs are IgG1...

  11. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...assays Multiplex assays involve multiple antibodies, each with a different signaling molecule, that all need... Rachel Leeson...

  12. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...lysogenic. Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into...bacterium to make a small “bridge” or sex pilus that attaches to the recipient cell drawing it close. Once in...

  13. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...communication specialist, Rachel, to write. Identify expertise While Rachel had antibody experience, ...they have expertise. Other times, we may need to reach out to an external expert to help us create accurate...about! You can also suggest blog topics or even reach out about writing a guest post (email [email protected]...

  14. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...that they’ll be compatible with your instruments, each other, and your target. I love FPbase’s Spectra ...Alkaline Phosphatase (AP). Various substrates for each of these enzymes allow you to detect enzyme activity...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...al., 2015). However, there are certainly other approaches that involve modifying the antibody sequence ...

  15. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of... If you want a plasmid for free, you can always reach out to the PI or corresponding author of the paper...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...

  16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...systematically mutated it. Using a robotic picking approach to select cells with variant characteristics they...” vector cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest ...2016)). During Agrobacterium-mediated DNA transfer, each GOI is usually expressed from a different plasmid...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...

  17. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...reproducibility workshops as Director of Scientific Outreach at Code Ocean and through her previous work at...Generating material to empower others to practice and teach reproducibility An early milestone for the group...instruct the workshop at the conference, but because each contributor made the materials accessible, anyone...were assessed and could improve over time. They achieved this by administering pre- and post-workshop surveys...

  18. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...scientists actually loving the MTA?! Visibility and outreach Scientists know about Addgene and our visibility...increasing internationally, thanks to evolving outreach tactics, increasing business development efforts...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...finishing your thesis as it is for helping your team reach an important milestone at work.   While I was in...always found that taking a conscious or active approach can be hugely helpful not only for developing ...can start by paying attention to how your mentors reach solutions and ask them to explain their logic. Or...about technical know-how and more about how you approach new challenges.     Know your strengths! Research...

  20. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...intended edit further weakened recognition by MMR machinery and improved editing efficiency. The lab also ...
Showing: 281 - 300 of 816 results