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We narrowed to 682 results for: eng

Showing: 281 - 300 of 682 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...fluorescent as well as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging...biosensors? Well, a lot of these sensors are already engineered into viral vectors, so if you want to use them...Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience...

  2. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...and tractable tools for genome engineering. Scientists have engineered viruses to target specific cell...infect. In the lab, this means viruses can be engineered to be cell type specific. Once inside the host...

  3. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...pGGA008 (AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light... induce transcription of your target gene? The strength of inducible promoters can vary a lot. If you’...

  4. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...reacting to a chemical, but by emitting a specific wavelength of light in response to excitation by a laser... degrading them. You’ll also need to know the wavelength of laser needed to excite them - and it’s a good...   Fig 2: Try to inversely pair fluorophore strength with protein abundance.    Second, you’ll ...

  5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...Non-Continuous Evolution (PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems...ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution ...Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR (2020) Phage-assisted...

  6. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

  7. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...advantage to using the CRISPR/Cas system for genome engineering is the fact that Cas9 can be easily programmed...smegmatis (Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair...UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence...

  8. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...excitation wavelength. A fluorophore is less likely to bleach when it is excited off the peak wavelength, since... excitation powers are often used to reduce the length of the experiments. As the photobleaching rate ...

  9. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... evaluate degradation of ENL. Cell lines were engineered to express ENL-FKBP12F36V and endogenous ENL ...evaluate degradation of MELK. Cell lines were engineered to express FKBP12F36V-MELK and endogenous MELK... evaluate degradation of YY1. Cell lines were engineered to express YY1-FKBP12F36V using CRISPR/Cas9-mediated...

  11. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...injected with an immunogen. This might be the full-length protein that you want to generate antibodies against...Otherwise, you might experience variability in the strength of staining and background noise. If you do use...control across experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher...

  12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...but the website looks interesting and it is in English and Spanish. She is based in Spain. The bottom ...

  13. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...its ground state by emission of a photon with wavelength specific to the acceptor In order for FRET to...quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage...Nature 388.6645 (1997): 882. PubMed PMID: 9278050. 3. Meng, Fanjie, et al. "A fluorescence energy transfer‐...

  14. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...high-complexity reactions? The researchers over at New England Biolabs® thought otherwise! They suspected a data-driven... Golden Gate Assemblies. Image courtesy of New England Biolabs.   DAD Tools The three DAD tools used...Temperature Incubation time/cycling Overhang length T4 DNA ligase 25 °C 1 hour 3-base or ...

  15. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...literature! PubMed PubMed is the classic search engine for finding papers. It’s been around since 1996...Zuckerberg Initiative. Meta is not a traditional search engine. Instead, you create your own research feed by ...

  16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...tool to the CRISPR tool box.  Nanoblades are engineered murine leukemia virus (MLV)-like particles loaded...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

  17. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...editing. LbCpf1 and AsCpf1 are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than...deletions at the 3’ end of the target sequence. Cpf1’s strength may lie in its low off-target editing rates, determined...

  18. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...fiber optics needed for optogenetics. First, the length of the fiber optic for the implant depends on the...started, you will need a laser of the appropriate wavelength to stimulate your opsin. Each opsin has an excitation...fiber optic patch cable must be the appropriate length for your specific behavioral apparatus or test ...

  19. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... Steven Jacobsen and Jennifer Doudna’s labs engineered the tobacco rattle virus (TRV), a bipartite RNA...tissue tropism for gene therapy and ex vivo cell engineering applications and is especially well-suited for...perform neuronal tracing, anterograde tracing poses challenges, from non-selective anterograde transport to ...

  20. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...scientists work to invent novel techniques or to engineer improved tools for familiar applications. But ...variants of MagLOV will be possible with other engineering approaches and that magnetosensitive tools can...biophysics, as well as inventors and biomedical engineers, join efforts to explore the mechanisms and develop...
Showing: 281 - 300 of 682 results