We narrowed to 513 results for: gats

...proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the low sensitivity...Interactions through Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed...

... to be replicated. Because E. coli are easily propagated and have a generation time of around 20 minutes...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

...misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...other model organisms. Worms have been used to investigate an array of biological systems and processes ...

...several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration is the most common analytical...protein of interest usually outweigh potential negative consequences of the tag. Many thanks to our guest...

...introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of ...Mouse Model of Limbal Stem Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): ...

... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...

...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...

...involved in endoplasmic reticulum quality control navigate a large and interconnected system of dynamic and...most prevalent inherited diseases. The authors investigated the role of cytosolic ubiquitin ligases in the...

...population-wide (as opposed to single-cell) approach is to interrogate state with qPCR. The re-arrangement of DNA segments... in each state. The three methods of state interrogation mentioned above (Sanger sequencing, qPCR, and...

...talent, of human knowledge, that has ever been gathered at the White House - with the possible exception...someone to coordinate, someone to decide, someone to delegate, etc. If you find that there is not someone (or...

...adequate expression is reached you may obtain a false negative or conclude the viral vector is not working.  ...expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. ...

...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...

...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...

... developing recombinant antibodies, antibody conjugation, chimeric antibodies, and smaller engineered ...engineered antibodies, including chimerization, conjugation, and antibody fragments. Fab: fragment antigen...

...Drugs, DREADDs) and optogenetics as tools to investigate the roles of certain cell types in locomotion...cause the least amount of immune response. Any investigator who is using an in vivo animal model should ...

...antibody.Note: Always include a positive and a negative control for the tagging experiment and test them...PubMed Central PMCID: PMC5312033. 2. Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn...

...at crossing the cell membrane. So, you should investigate your options and weigh the potential benefits...). In vivo protein labeling with trimethoprim conjugates: A flexible chemical tag. Nature Methods, 2(4...

... create a thin sheet of excitation light that propagates perpendicular to an imaging objective that collects...excitation light is completely reflected, energy is propagated into the sample via an evanescent wave that only...

...targeting each of the 1000 genes you’d like to investigate in your next CRISPR screen. Luckily, the Green... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...

... intermediate pluripotent state is not always obligatory in cell conversion processes [35]. Rather than... during the reprogramming process as they are propagated in vitro as part of the process. This opens up...