We narrowed to 415 results for: genome

... 3, and of course the rapidly changing world of genome engineering via CRISPR.  Though the individual ... a thing if CRISPR hadn’t made manipulating the genome cheap and relatively easy? Have all of these things...

...be delivered to cells both transiently and for genomic integration. Lab-engineered viruses will package...package your genetic cargo in lieu of their own genomes and deposit this cargo upon host infection. Packaging...

...the vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying...

... and her aim is to produce constructs and edit genomes so as to make plants, and agricultural crops in...working on a lot of CRISPR tools for editing plant genomes. We will definitely plan to share those tools with...

...knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we receive...: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read ...

...check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve’s lab ...

... Biogen interested in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR...

...-stranded intermediate into the single-stranded genome that's then packaged into AAV’s protein shell, ...: AAV titering by qPCR quantifies the number of genome-containing viral particles that are present in ...

... the genome. A Cas13-based RNA editing system would likely be reversible and would avoid genomic off-targets...

...CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term ... it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral genomes (vg) per mouse. Figure 3 shows a gross comparison...

... FURIN, and more. Recently, scientists used the genome-wide CRISPR libraries, Brunello (knock out) and...Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins...

... sites, the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left...fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of organisms ...

...with over 700 views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science...

...purity testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged...

...Context-Dependent Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune...

...system to integrate particular genes into mammalian genomes and easily disrupt gene expression to study gene...TLE/Grg family of transcriptional co-repressors." Genome biology 9.1 (2008): 205. PubMed PMID: 18254933....

...which can become costly and impractical for whole-genome (and often, whole-plasmid) sequencing.  Prior to...well as the ability to sequence an entire viral genome in one read. We also use Nanopore sequencing to...

...cells), or they can also be integrated into the genome by cotransfecting them with a plasmid expressing...Lett. 110, 073704 (2017). Hurt, R. C. et al. Genomically mined acoustic reporter genes for real-time in...

...alone, and this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now...22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial plasmids: classification and identification...

...that do not involve modification of the original genome by integrating viral vectors are preferred. This...which is the integration of transgenes into the genome that can cause disruption of functional genes at...