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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 411 results for: genome

Showing: 281 - 300 of 411 results

  1. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we receive...: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read ...

  3. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... Biogen interested in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR...

  4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... FURIN, and more. Recently, scientists used the genome-wide CRISPR libraries, Brunello (knock out) and...Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins...

  5. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... the genome. A Cas13-based RNA editing system would likely be reversible and would avoid genomic off-targets...

  6. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... sites, the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left...fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of organisms ...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...-stranded intermediate into the single-stranded genome that's then packaged into AAV’s protein shell, ...: AAV titering by qPCR quantifies the number of genome-containing viral particles that are present in ...

  8. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term ... it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral genomes (vg) per mouse. Figure 3 shows a gross comparison...

  9. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...with over 700 views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science...

  10. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...purity testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged...

  12. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...which can become costly and impractical for whole-genome (and often, whole-plasmid) sequencing.  Prior to...well as the ability to sequence an entire viral genome in one read. We also use Nanopore sequencing to...

  13. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...cells), or they can also be integrated into the genome by cotransfecting them with a plasmid expressing...Lett. 110, 073704 (2017). Hurt, R. C. et al. Genomically mined acoustic reporter genes for real-time in...

  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...alone, and this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now...22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial plasmids: classification and identification...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...  IPTG-inducible expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ:...expresses T7 RNA polymerase (RNAP) from the bacterial genome under control of the lac repressor, which is inducible...

  19. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. Ahmed A. Gomaa, et...
Showing: 281 - 300 of 411 results