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We narrowed to 745 results for: nes

Showing: 281 - 300 of 745 results

  1. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...miss out on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela DePace...lab culture. The aim of these meetings is to give honest, constructive feedback, set tangible research and...personal development through thoughtful planning and honest feedback DePace’s yearly planning meetings revolve...person organize his or her thoughts to facilitate honest conversation. Goals and Planning Worksheet: ...

  2. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ... more often than other disciplines. The majority of tweets for most disciplines contain scientific domains...#8. Figure 1: Highly Represented Scientific Disciplines on Twitter. Twitter users were identified as ...Follower networks of subgroups of scientific disciplines: social (left), life (center), and physical (...

  3. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce irreversible...another important step forward by dissecting that S. pyogenes protein into three parts. Their efforts yielded... from the “S” in Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together... chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this purpose?...

  4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...into the following categories: Over 300 empty backbones with 100+ distinct fluorophores to choose from...constructs for biosensing. Over 300 plasmid backbones spanning a rainbow of fluorescent proteins “It...It’s a great collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working...collection with such variety.” In most cases, backbones are available to attach the gene of interest to...

  5. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ... Encodes insert(s) A plasmid with one or more genes, protein coding sequences, or other elements inserted...designed for cloning a gene/insert into it. Empty backbones often contain epitope tags or fusion proteins ...Insert *Note: This page will not appear for empty backbones Please enter the name of any gene contained within...Addgene ID in the "Backbone Manufacturer" box for backbones requested through Addgene. For example, you could...Addgene qualifies as BL1 or BL2 as defined by NIH guidelines. I hereby certify that I have disclosed all known...

  6. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...all know that the Fc domain provides structure, determines isotypes, and provides a place for secondary ...cannot survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...induced “stabbing” and “poisoning,” but let’s be honest, the elimination style the immune system is most... that!    References and resources References Clynes RA, Towers TL, Presta LG, Ravetch JV. Inhibitory...

  7. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...two genes: the replication (rep) gene and the capsid (cap) gene. In wild-type AAV, these two genes are...which contains the AAV structural and packaging genes, the adenoviral helper plasmid which contains the...Next let’s take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products...

  8. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...currently houses over 890 CRISPR screens in 629 cell lines, each manually curated and annotated from more than...and power of CRISPR-based library screens in cell lines from humans and other species. By accurately compiling...browse through screens performed in relevant cell lines or conditions. This increased ease of access not...screens performed in 26 different breast cancer cell lines. Screenshot updated 2024. Each screen curated...

  9. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...Giving honest and helpful feedback gets easier with practice so you must build it into your routines. This...their work.  Check out Joanne's Reddit AMA   Guidelines for giving feedback Use formal and informal ...differently? Beware of indirect language You must be honest about performance problems. While giving corrective...

  10. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...capable of robust expression of high G+C content genes, as validated through monitoring the expression ...adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...pooled library from Junjie Chen's lab targets human genes known or suspected to be involved in DNA damage ...

  11. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture ...production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et al., ...hybridomas can lose gene expression of the mAb-encoding genes, die, or fail to revive after cryopreservation (...Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, ...

  12. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Kamens several years ago through the Healthcare Businesswomen’s Association and it was through this connection... systematically test the function of all 20,000 genes in disease models and then focus drug discovery ...early age, I was fascinated by the impact that medicines can have on human health. As a teenager in high...the field of gene therapy.” He’s also worked on vaccines throughout that time. Now Vandenberghe is an Associate...

  13. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...raw plasmid sequence and display frequently used genes and control elements. Development of this tool required...feature sequences due to genetic drift and the use of genes from different microbial strains. It proved to be...sequence feature that may be used to make fusion genes, detection needs to occur even if one or two codons... our database, or if you see an opportunity to harness common feature detection in a new way, please contact...

  14. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ... Addgene to gene-edit mutations into XP genes and other genes involved in photosensitivity in melanocytes...hemiplegia) that are often painful and accompanied by migraines. Those attacks can be brought on by a number of...plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying different SOX10 mutations...

  15. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... cell type of interest better than immortalized lines derived from the same cell type; however, their ...interest into primary cells, so most primary cell lines require viral infection. A new paper from Niels ...alter protein function or localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis...biallelic mutations. Importantly, hESCs retained their stemness, indicating that iTOP may be valuable for CRISPR...

  16. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... delete/overexpress endogenous genes or to introduce heterologous genes, but the trend of research has...mimic natural transcriptional programs on multiple genes. Modifying the CRISPRi system for multidirectional...programs to differentially alter the levels of five genes that encode biosynthetic enzymes for violacein, ...

  17. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...screens, enabling genome-wide interrogation of the genes required for cellular processes. For a successful...that constitutively express Cas9 and screen for genes that affect Th2 differentiation and activation, ...-LPs and accelerate the discovery of additional genes and small-molecule probes to aid in the understanding...to different lipid compositions of C. elegans membranes, which might beneficially influence the rhodopsin...

  18. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...medical conditions had been swapped with made up illnesses. They were then asked to evaluate the quality ...methodology makes it easier to spot even minor weakness or limitations in a piece of research (Drummond... mask designs could work to slow the spread of illness. I later helped run workshops where I showed people... jargon that even scientists in neighbouring disciplines might struggle to understand. Why should we trust...

  19. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...keeping the system to minimal size. To do so, they harnessed the tRNA processing mechanism, which is conserved...lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases, such as muscular...for correcting mutations or installing functional genes to study and potentially treat polygenic diseases... that would perturb the unprecedented number of genes with a single viral vector.   Fig. 1: Schematic...

  20. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...assignment to discuss goals, expected outcomes, timelines and deadlines. Agree on a way to review project progress...identify constraints and boundaries. Where are the lines of authority, responsibility and accountability?...try "Why Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation tips...
Showing: 281 - 300 of 745 results