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We narrowed to 507 results for: otos

Showing: 281 - 300 of 507 results

  1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ... incubated together to form the RNP. Detailed protocols outlining these steps have been made publicly ...Polyethylene Glycol (PEG) mediated transfection of protoplasts and biolistic bombardment of immature embryos...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted... time, etc., which can vary even when the same protocol is being followed.  Finally, standard curves must...

  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...across systems and species. The cost? Zeocin is genotoxic when not 100% inhibited, meaning it will induce...Addgene.org Addgene’s Basic Molecular Biology Protocols Over-Agar Antibiotic Plating Plasmids 101 eBook...

  4. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...adenovirus type 5 (hAdV-C5) can be considered the prototype, although many viruses infecting humans (from ...oxfordjournals.aje.a113563. PMID: 6301263. Servellita, V., Sotomayor Gonzalez, A., Lamson, D. M., Foresythe, A., Huh...

  6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...that increased the fluorescence intensity and photostability of GFP. This also shifted its major excitation...Known Function S65T Increased fluorescence, photostability, and a shift of the major excitation peak to...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and Resources More Lentiviral FAQs Answered by...

  10. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... for Protein Expression in Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed...Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic Restriction Digest Video ...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics. Several...

  13. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...antibody new tricks By Ashley Waldron While IHC protocols typically call for thin sections of tissue, the...perform well in standard IHC and western blot protocols. You can find more specific information on the...

  15. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...virus properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 7....

  17. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

  18. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...Secreted Proteins: The most common purification protocols are designed for soluble, cystosolic protein products... can be recovered and purified using a special protocol. Alternatively, reducing the concentration of ...

  19. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science...University. She is a scientist with a passion for photosynthetic communities, microbial interactions, and science...

  20. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...
Showing: 281 - 300 of 507 results