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  3. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... for Protein Expression in Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed...Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic Restriction Digest Video ...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...antibody new tricks By Ashley Waldron While IHC protocols typically call for thin sections of tissue, the...perform well in standard IHC and western blot protocols. You can find more specific information on the...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics. Several...

  7. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...virus properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 7....

  9. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

  10. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...Secreted Proteins: The most common purification protocols are designed for soluble, cystosolic protein products... can be recovered and purified using a special protocol. Alternatively, reducing the concentration of ...

  11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...

  12. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science...University. She is a scientist with a passion for photosynthetic communities, microbial interactions, and science...

  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...the application of this approach is limited to protoplast cells (plant cells whose cell wall has been removed...recommend reading the associated publications or protocols to get the most out of the plasmid you chose for...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...activity. Researchers have designed a variety of photoswitchable elements into many other types of proteins.... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...Lentiviral Guide and our lentiviral production protocol.  One thing to keep in mind is that titering these... these IDLVs may require a slightly different protocol than regular lentiviral vectors. This is because...

  17. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...Find other tips and tricks for molecular biology protocols Browse other articles related to antibodies Resources...Resources on Addgene.org Find written and video protocols Visit Addgene's antibodies collection ...

  18. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... Require Ex Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002... CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002...

  19. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...protein to a DNA-editing enzyme to generate the prototypical base editor enzymes. The cytidine base editors...engineering using the CRISPR-Cas9 system. Nature Protocols 8:2281–2308 . https://doi.org/10.1038/nprot.2013.143...

  20. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment...Activated By Designer Drugs) to determine the role of serotonin in movement. Follow her on twitter @katrinaboards...
Showing: 281 - 300 of 495 results