Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 628 results for: rel

Showing: 281 - 300 of 628 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...Within the animal kingdom, this information is relayed through factors (both of endocrine and dietary ...systematic effort to compile NR- and coregulator-related global gene profiling datasets in to our data mining...coregulators. We look forward to growing this important relationship with Addgene for the benefit of the entire research...

  2. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...fluorophore--think of it as a virtual photon 3. Relaxation of the acceptor fluorophore back to its ground...donor-acceptor pair and a change in FRET efficiency correlates with a change in the distance and/or orientation...simultaneously would further increase the amount of correlated information available from a cellular process...

  3. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...terminals (Lafon 2005). Therefore, once RABV is released from an infected cell, it is only taken up by ...genome). Nascent G protein-coated RABV is then released from the starter cell and transmits retrogradely...mediated by other viral proteins), but the newly released RABV cannot infect any subsequent cells due to...

  4. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ... HR carefully because sometimes companies have relaxed dress codes for interviews. You can also ask HR... know up front how you plan to take questions.  Relax!  You wouldn’t have made it to the interview if ...position. So prepare for the interview, but then relax.  Additional resources on the Addgene blog: Tips...

  5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...   Epigenetics nation stuck with methylation correlation  In the 1980s, a lot of things were cool: Walkmans...problem is that many of these studies report correlations without clearly testing whether a certain methylation... dCas9-TET, allowing one to study the causal relationship between DNA demethylation and gene expression...

  6. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...early-phase clinical trials and have shown promising preliminary results (Dimitri et al). CRISPR engineering of...patients experiencing sustained and substantial relief from both disorders. As of publication, submissions...The future success of CRISPR in the clinical will rely on improving and evolving better CRISPR systems ...

  7. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ..., give it back to you, and for you to make the relevant alterations before submitting - handing them your...in your research proposal are originality and relevance. When reading through your proposal, ask yourself...your work, or even the successful use of your preliminary data to secure further funding. Make it clear...

  8. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...major advances in light microscopy, particularly related to the neurosciences.  These developments in microscopy...microscopy) (16).  Both 3DISCO and CLARITY have relied on the use of transgenic mice in which a subpopulation... our understanding of the structure-function relationship of the central nervous system under normal and...

  9. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...near the beginning or end of a reaction is often unreliable, we suggest using the “Somewhat similar sequences... that you can extract practically every single reliable base pair from your results. This option is not...searches Once you have used blastn to determine the reliable portion of a Sanger sequencing result and noted...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...For a breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's post from last week...not narrow enough to distinguish between closely related colors. Furthermore, most FPs  have a broad range...College of Medicine. He is interested in everything related to gene expression, particularly at the RNA level...

  11. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... terms used, so you will only be shown filters relevant to your current search. We’ll take a closer look...and filters change as we now only show options relevant to your current search terms and potential results...helpful information about the subject, link to related guides and protocols, and provide easy to sort ...

  12. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...widely used DNA sequencing technology. The process relies on “sequencing-by-synthesis,” where each base is...sequencing.  Prior to short-read sequencing, Addgene relied on Sanger sequencing for QC. We occasionally still...Second-generation sequencing”)  Short-read sequencing also relies on sequencing by synthesis, but modifies the original...

  13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...Several different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2... that coordinate development of the body plan (Farrell et al., 2018). This study also showed that in the...Nature 568.7751 (2019): 193. PubMed PMID: 30944477. Farrell, Jeffrey A., et al. "Single-cell reconstruction...

  14. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... expand as Rett scientists deposit their MECP2-related plasmid tools with Addgene and share additional...Methyl-CpG-binding protein 2 mutations and phenotype-genotype correlations. Am J Med Genet 97:147–152 . https://doi.org...Determines the Clinical Course of Rett Syndrome and Related Disorders. Cell 152:984–996 . https://doi.org/10.1016...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...usage important? Translation and protein synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver...studies have shown that translational efficiency is correlated with codon bias across all endogenous genes in...you want to express may contain codons that are rarely used in the host you are using or come from an ...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...session is to start with something completely unrelated to the issue and consider how it could be shaped...encouraging dissent, the next tip may be particularly relevant to you. Elect a devil’s advocate. This is the ...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

  17. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... years later. While there is a dose-response relationship (the higher the exposure, the greater the risk... year). Research efforts in mesothelioma instead rely upon private donations. Many people think that because...exposed even one time. Doctors and researchers don’t entirely understand why some people develop the disease...

  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...provide many opportunities. But these continue to rely on printed journal articles as the dominant tool...economics, or maybe even biology or international relations.  Many of the challenges in science today are ...popular biology videos are:     “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing...

  19. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...proper controls are invariably inconclusive and unreliable. Proper controls provide the constant variables...Regardless, the amount of reporter protein activity correlates to both the amount of DNA transfected into the.... Replicate controls: technical and biological Reliable results are reproducible from properly controlled...

  20. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...can do and, in this way, science has always been related to government and, consequently, politics. More...Inclusion of data informing policy specifically related to the following subjects: Climate change Vaccines...Healthcare Science education To make scientists relatable (17, 20). This is an effort to show the general...
Showing: 281 - 300 of 628 results