...near the beginning or end of a reaction is often unreliable, we suggest using the “Somewhat similar sequences... that you can extract practically every single reliable base pair from your results. This option is not...searches Once you have used blastn to determine the reliable portion of a Sanger sequencing result and noted...

...For a breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's post from last week...not narrow enough to distinguish between closely related colors. Furthermore, most FPs  have a broad range...College of Medicine. He is interested in everything related to gene expression, particularly at the RNA level...

... terms used, so you will only be shown filters relevant to your current search. We’ll take a closer look...and filters change as we now only show options relevant to your current search terms and potential results...helpful information about the subject, link to related guides and protocols, and provide easy to sort ...

...Several different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2... that coordinate development of the body plan (Farrell et al., 2018). This study also showed that in the...Nature 568.7751 (2019): 193. PubMed PMID: 30944477. Farrell, Jeffrey A., et al. "Single-cell reconstruction...

... years later. While there is a dose-response relationship (the higher the exposure, the greater the risk... year). Research efforts in mesothelioma instead rely upon private donations. Many people think that because...exposed even one time. Doctors and researchers don’t entirely understand why some people develop the disease...

...session is to start with something completely unrelated to the issue and consider how it could be shaped...encouraging dissent, the next tip may be particularly relevant to you. Elect a devil’s advocate. This is the ...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

... expand as Rett scientists deposit their MECP2-related plasmid tools with Addgene and share additional...Methyl-CpG-binding protein 2 mutations and phenotype-genotype correlations. Am J Med Genet 97:147–152 . https://doi.org...Determines the Clinical Course of Rett Syndrome and Related Disorders. Cell 152:984–996 . https://doi.org/10.1016...

...usage important? Translation and protein synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver...studies have shown that translational efficiency is correlated with codon bias across all endogenous genes in...you want to express may contain codons that are rarely used in the host you are using or come from an ...

...provide many opportunities. But these continue to rely on printed journal articles as the dominant tool...economics, or maybe even biology or international relations.  Many of the challenges in science today are ...popular biology videos are:     “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing...

...proper controls are invariably inconclusive and unreliable. Proper controls provide the constant variables...Regardless, the amount of reporter protein activity correlates to both the amount of DNA transfected into the.... Replicate controls: technical and biological Reliable results are reproducible from properly controlled...

...can do and, in this way, science has always been related to government and, consequently, politics. More...Inclusion of data informing policy specifically related to the following subjects: Climate change Vaccines...Healthcare Science education To make scientists relatable (17, 20). This is an effort to show the general...

...Many intracellular sensors of events like calcium release have been made using fluorescent resonance energy...biosensors, the intensity of the FRET signal can be correlated to specific concentrations of biological molecules...et al. (7) wished to trigger intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring...

... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...stay around? Is it too long or do they leave prematurely? Do lab alumni stay in the same field taking...Does the PI keep up on the literature and pass on relevant papers? Will you have access to information from...

...ligase, conditions and suitable probes, LDR can reliably detect single-base differences. The original creators...interrogation of multiple potential SNP sites (15). The related LCR technique uses two sets of probes to achieve...DNA library preparation. However, when a method relies on accurate ligation of nicks lacking gaps or mismatched...

... give advice and guidance to those that ask. Relationship building is key in science policy. Luckily, ...happy hours that you can attend to help build relationships.  Reach out to a science policy professional..., communications, diplomacy and international relations, advocacy, and writing legislation (see Figure...

...for dinosaurs wreaking havoc in a theme park.  Released in 1997, GATTACA is an American SciFi film starring... breaks (DSBs) in the genome. These technologies rely on the cellular DNA repair machinery, which can ...the adenine base editor (ABE) enzyme in 2017. ABEs rely on a similar strategy as CBE but use a ssDNA adenosine...

...vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic liposome) or electrical...Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant and...viral capsid antigen p24, the FACS dosing method correlates viral titre to the number of cells transduced...

...Biochemist, we geneticists are a lazy bunch who love to rely on the awesome power of genetic selection. Knock-in...recovery of these edits. Initial work in C. elegans relied on selective markers such as drug resistance (Chen...geneticist, I developed a co-selection approach relying on repair of a conditional-lethal mutation to identify...

...2011. 12(2): p. 79-89. PubMed PMID: 21252997. 37. Merrell, A.J. and B.Z. Stanger, Adult cell plasticity in...2016. 17(7): p. 413-25. PubMed PMID: 26979497. 38. Thorel, F., et al., Conversion of adult pancreatic alpha-cells...Models for Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem...

...assembled or theoretical — it does not have to be entirely verified by sequencing. Upload any support files...easier for other scientists to find information related to your plasmid. It is optional to include the ...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...