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We narrowed to 512 results for: sam

Showing: 281 - 300 of 512 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...system to GAL4/UAS that functions in essentially the same manner, with LexA binding to the lexA operator (...desirable to control or look at multiple genes at the same time.  The 3 binary systems listed above are orthogonal...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...Watson-Crick base pair, are joined with nearly the same efficiency as a correct base pair. DNA ligases have... resistance-mediating polymorphisms in African samples of Plasmodium falciparum." Journal of clinical ...

  5. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...ribosome entry site (IRES) and expressed under the same promoter. Alternatively, your gene and the marker...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ... about these methods is that they all follow the same general cyclical process (Figure 2). The differences...codons (different codon sequences that code for the same amino acid) for various amino acids. Essentially...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...validation screen using selected gRNAs extracted from the same or another library. These different approaches could...Listed you can also easily extract gRNAs for the same gene from several libraries, and identify those ...

  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ... library is a set of plasmids all built with the same backbone and only differing in the gene/insert. ... Pro Tip! If you have multiple plasmids with the same gene or vector backbone, you can copy some information...

  11. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    .... This can complicate comparisons made between samples, i.e. transgene expression and hence the extent...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ... similar to using a single chimeric gRNA. At the same time, the off-target effects are reduced because...genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the Science paper (Cong et al...

  14. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...find out the final four! (Note: For cases where the same name was submitted by more than one person, we selected...

  16. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...I was bored this weekend while waiting to take samples from my assay at like 23:00, so I decided to make...

  17. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ... using the link or use the text below to get the same information. Download a PDF of CRISPR Cheat Sheet...
Showing: 281 - 300 of 512 results