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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 459 results for: try

Showing: 281 - 300 of 459 results

  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015). Nevertheless, the use of these...visualizing motor, visual, and auditory neuronal circuitry in vivo. A variant of RABV has been developed ... allowed for detailed mapping of the neuronal circuitry involved in learning and memory (Kohara et al....PMID: 21825165. PubMed Central PMCID: PMC3174680. Buttry, J.L., and Goshgarian, H.G. (2015). WGA-Alexa Transsynaptic...

  2. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...visualization of specific proteins in situ immunocytochemistry is perhaps the most common approach. With...students at the Max Planck Institute for Biophysical Chemistry, Göttingen in Germany. I attended this symposium... labeled proteins can be detected using click-chemistry that covalently links the azido containing AHA...

  4. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...

  5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’... (i.e. RNA replication with no proteins). The chemistry is complex and this work requires an interdisciplinary...forms as we shared millions of years of common ancestry with worms before evolutionary divergence. Nikolaus...

  6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

  8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...can be easily modified in order to facilitate its entry into the target cells (Beatty et al., 2012). These...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/...

  9. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...machine learning to solve difficult problems in chemistry and chemical biology.       David R. Liu is Director... Faculty at the Broad Institute; Professor of Chemistry and Chemical Biology at Harvard University; and...Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate biology and enable...

  10. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm. In addition to the...forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated...electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215. PubMed...

  11. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable development..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...those conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech,...about the trends and workings of the healthcare industry. Finally, on-campus resources like career offices... both provide up-to-date information about the industry, recruiting processes, and can connect you to ...

  13. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ... chemically pre-treating the cells to allow for entry of the antibodies. Here, a two-step process is employed.... The main applications of dye stains in flow cytometry are cell proliferation and cell viability. Unlike...101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

  14. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... unconjugated and recommended for use in flow cytometry. Based on the established applications of the ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...

  15. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...more stable yet brighter furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... populations of synapses and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4...immunoprecipitation (IP), and tandem mass tag (TMT) mass spectrometry (MS) in HCT116 cells (colorectal cancer). The...isolated and identified with tools like mass spectrometry, providing a snapshot of interacting proteins...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...contributions to this work. Søren Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve...

  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing...expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA ...

  20. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...serotype, promoter, or tool type. Drilling into any entry will reveal additional data including the subject...injection before performing the experiment. Every entry has an accompanying image so that you can see for...
Showing: 281 - 300 of 459 results