We narrowed to 599 results for: REV;
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TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....sugar-containing molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology... have visible colonies growing on the membrane wherever an E. coli colony expressing a functional binding... References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications for old resources...Bases in Bacteria and Their Viruses." Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David...
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A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m....everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...or not. Choose wisely This point ties into the previous one too. I would recommend applying for multiple...factors that can make or break a lab experience. Previous Addgene blogs have discussed the process that ... but I’ve learned the value in finding mentors wherever I go. This story by Dr. Erika Moore in Science... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....yeast, flies, and even plants! In this blog we will review common uses of MoClo and the kits Addgene offers...the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015). This kit...2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression, ...Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m.... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...suitable for targeting FRET acceptors to proteins (reviewed in [Yan & Bruchez, 2015]). To get you started,...HTRF: a technology tailored for drug discovery–a review of theoretical aspects and recent applications....energy transfer as a spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....of plasmid prep do you need? As we covered in a previous post on plasmid preps, not all plasmid preps are...purification kits make it easier than others to prevent quality issues. For example, some kits reduce the...weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore... high level of consistency between lots, then reviewing their quality assurance documentation can help... -
Plasmids 101: How to Name Your Plasmid in 3 Easy Steps
TypeBlog PostPublishedFeb. 13, 2014, 1 p.m....case letter to the beginning of their insert abbreviation to specify what species it is. Example: ‘h’ ...amino acid mutation is to list the one letter abbreviation of the wild type amino acid immediately followed... Methionine (Met) followed by the one letter abbreviation of the mutated amino acid currently at that ... -
Top 10 Open Science Developments of 2013
TypeBlog PostPublishedDec. 19, 2013, 3:46 p.m....Annals of Internal Medicine published systematic reviews on an agent used to promote bone growth based on... open access as it does about the state of peer review. 8) That same issue of Science also included...showing overwhelming support for open access to peer-reviewed journal publications, although many don’t necessarily... -
Celebrating Accomplishments in the Lab
TypeBlog PostPublishedMay 8, 2014, 3:07 p.m....recent conversations with three lab heads have revealed that a combination of hard work, determination...How do you manage work-life balance? Tom Ellis reveals “I don't. Ask me again in five years and hopefully...Scientists Maintain a Good Work-Life Balance? Read the Previous Posts in this Series Advice for Choosing a Research... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog PostPublishedJuly 20, 2017, 2:30 p.m.... et al. "PP09. 1–2352: Systematic review of incidence and prevalence of spinal muscular atrophy (SMA)....S65. Additional Resources on the Addgene Blog Previous BeHeard Awardees Other Efforts to Support Rare... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog PostPublishedOct. 15, 2024, 1:15 p.m....be completed and reviewed. The timeline therefore will be as follows: Request review: 1–2 days Prep and...µL (5 mL). Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...