...Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient cells during a tri-parental...RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the ability to synthesize diaminopimelic...your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M....

... specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, ...epitope recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide...., who published a paper showing that the amino acids neighboring the Myc epitope influence 9E10’s affinity...Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...

...Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about...questions and alternative systems The ready-made PITCh plasmids available from Addgene are perfect for expressing...Protoc. 11(1): 118-33. PMID: 26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, ...” Cell Rep. 13: 621-633. PMID: 26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi...about CRISPR on Our CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid ...

...compared to transgenic animals (17).  These viral plasmids are available through Addgene.  The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References: H... Ross A, J. Hecksher-Sorensen, R. Baldock,  D. Davidson. (2002) Science, 296, 541-545. Pubmed. A. Ertürk.... Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et al. (2013) Nature, 497, 332-337. Pubmed. R....

...MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry applications...modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple cloning...throughput cloning is to facilitate the construction of plasmids with many components. The creators of the MXS-...Assembly Learn About Other Plasmid Cloning Techniques Plasmids 101: Green Fluorescent Protein Resources on Addgene.org...nearest neighbor base sequences in deoxyribonucleic acids. The Journal of biological chemistry. 1962. June...

...can be encoded in the primer sequences. Multiple plasmids can be included in a single PCR, for example for... to the PCR mix. When PCR is not possible Some plasmids just won’t amplify! Examples of this are the GC-rich... GC-rich ITRs of AAV genomic DNA encoding plasmids, or promoters such as the chicken beta-actin promoter...cloning by homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID: 8346047...resources on the Addgene blog Read all of our Plasmids 101 blog posts Read our blog post on 5 factors...

...transformation Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...localization of your protein of interest. Find all plasmids from the Lee-Parsons lab. Test different leaf...

...mediated Genome Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038/mtna.2016.48 Katti...identify and enrich base editing activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/...APOBEC–Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar...editing enrichment (TREE) in human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

...mammalian species to produce gas vesicles. New mARG plasmids Our new mARGs come as a set of two PiggyBac integratable...genes gvpNJKFGWV marked with Emerald GFP. These plasmids can be used directly in transient transfection...several generations to dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction...set of transient constitutively-expressed mARG plasmids driven by CMV promoters. Labs that are new to ...which is the transient cotransfection of the mARG plamsids into HEK293T cells using a chemical transfection...

... an allelic exchange experiment? Find the pTOX plasmids here! Continued development of new allelic exchange...and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID: 24234441...systems for unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID: 25800749...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn...recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our molecular biology...

...Find Synthetic Biology Plasmids Find Bacterial Expression Systems Find Plasmids for Protein Purification...associated with phosphorylation. However, these amino acids fail to recapitulate the true steric and charge-... during translation. Many ‘flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked...2014: mutations, PTMs and recalibrations." Nucleic acids research 43.D1 (2015): D512-D520. PubMed PMID: 25514926...

...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

... labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus, we are...trends for Zinc Finger, TALE, CRISPR, and SynBio plasmids over time. You can see these data in the slide...molecular biology technology? Have you deposited your plasmids yet?      ...

... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to ...CyOFP1-NLuc-CyOFP1; Antares). Direct injection of plasmids for Antares or firefly luciferase into mouse livers...Additional Resources on Addgene.org Browse Luciferase Plasmids Browse the Fluorescent Protein Collection Find...

...thumb, we avoid target sites that code for amino acids near the N’ terminus of the protein, in order to...Likewise, we avoid target sites that code for amino acids close to the C’ terminus of the protein, to maximize...dCas9-mediated transcriptional repression. Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583...scarless human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...

...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles...

...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...

...quickly. There are over 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been...plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that have helped scientists... of the Addgene community. Perhaps you’ll find plasmids and new tools that will speed your science along...lab closures, we’re expecting to reach ~14,000 plasmids deposited this year once they all get processed...

...Christopher E. French. Dr. Duedu was recently awarded plasmids from Addgene as a successful applicant to the ... fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby...plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian...out our Microbiology Pages Find Synthetc Biology Plasmids Find Molecular Biology Resources ...

...phage pL promoter.  It is a good idea to choose plasmids that also express the repressors associated with...needed. This approach is best suited for modifying plasmids or BACs because it results in stable integration...restriction enzyme digest can be used to screen plasmids for the appropriate mutations.  Point mutations... Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods...Browse our Microbiology Pages  Find Lambda Red Plasmids and Strains Check Out Other Molecular Biology ...