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We narrowed to 838 results for: IGH@

Showing: 601 - 640 of 838 results

  1. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...trainees’ very way of thinking too. Albert Einstein might have put it best when he stated, “the value of an...pathways (precisely the type of exercise Einstein might have resented!). In 1997, Joseph Provost, then a...like an exciting opportunity for your classroom, I highly recommend you try implementing one. It's likely...

  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...standard curve for qPCR can be challenging to get right. Here at Addgene, we use a linearized plasmid standard...very few positive droplets. In the image to the right, there is approximately 1 positive copy per microliter...droplet generator to the PCR plate. Maintaining a high droplet count is important for calculating the concentration...

  3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ... #MolluscMonday: #Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is...an extreme number of nerve cells and an unusually high level of RNA recoding in the brain. RNA editing ...DNA in the eggs to get transgenic animals. They might be immortal. Manihot esculenta (Blake Meyers) Cassava...

  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...million dollar sales pitch. Given this large sum, one might expect that applicants would work for months to ...research? This is the central idea. It should be at a high level, broadly understandable to scientists outside...engaged by your presentation, then you’re on the right track. Furthermore, practice getting back to your...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...data inside the ELN is reproducible, and only the highly restricted number of people who can access the ...practical to move all this data in bulk into a lightweight tool like an ELN. It is nevertheless critically...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...particular gene. Furthermore, a target sequence may have high homology elsewhere in the genome. These off-target...Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome Biol 19...Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature 556:57–63 . https://doi....

  7. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...depositing lab. (For our more popular libraries, you might receive a similarly-sized aliquot from an amplification...ll want to infect your cells with the virus–the right amount of virus, that is. Ideally, each cell will...Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9...

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...interested audiences quickly and concisely – video might be part of the answer. Problems with written publications...TedTalks are very popular, though they focus mostly on highly innovative ideas rather than on the day-to-day ...that provides links to the videos, keeping the copyright and control over the videos with the researcher...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through...Other investigators who had been working to develop high efficiency gene targeting approaches improved on...repair, it has proven more challenging to develop high efficiency CRISPR-based approaches for the generation...

  10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    .... This over-reliance on the perfect story for a “high impact” publication can lead to the publication ...reproducibility issues in the biological sciences. I’d highly recommend listening to the panel discussion to ...Scientific Figures into Dashboards and Share Your Insights Who gives a tweet? 9 facts about scientists on...

  11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...may be a good choice for targeting neurons, with high transduction frequencies in the central nervous ... our in-stock viral prep service.  AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid...AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency, and is an excellent choice...

  12. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...important that your gRNA sequence is designed to be highly active and reduce off targets. For CRISPRa and ...consuming process and should not be undertaken lightly! Indeed, truly achieving the gold standard requires...genetic screens using genome-wide libraries have shown high concordance between different sequences targeting...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ... LITE system, which uses light to regulate transcription, also includes light-regulated histone methyltransferases... CA (2017) CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...using them. Indeed, the future of mouse work is bright as it is now easier than ever to manipulate the...technology, CRISPR, that is simple in concept, straightforward to use, and robust in performance. In its natural...BL, Xavier RJ, Root DE (2014) Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation...

  16. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime editing section...

  19. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various...

  20. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...resources! Ready to sing along with this year's lineup? Right! We're ready to, so let's get started!  On the...sequences🎵On the 2nd day of CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation...

  21. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...from the first generation of base editors to the eighth generation (and dual base editors, too!).  On the...gave to me: CRISPR methods for bacteria.  On the eighth day of CRISPR, Addgene gave to me: anti-CRISPR ...

  22. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape garland. Thanks @ribosholmes... bit of everything: frog snowflakes, ornaments, lights, and took a group photo wearing holiday sweaters...

  23. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ... In each short episode of this series, we’ll highlight one tip that we’ve learned in the lab that will...

  24. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene. A... The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598...

  25. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ... world making breakthrough discoveries!” I knew right then I had found my dream job, because one thing...latest technologies and breakthrough research. Insights about how to navigate through all stages of your...

  26. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create a plasmid...rap video for us (If you’re new to plasmids, we highly recommend checking out the video). Tom has made...

  27. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...every new lab mate? Do you have a trick to get the highest yield out of your minipreps? If so, we’d love to...Addgene Blog Possible guest topics Some topics you might consider are: An overview of software tools useful...

  28. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...each of the Hot Plasmids segments into its own (highly Tweetable!) audio clip. In the future, you’ll be...Domain Constructs Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera...

  29. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...account, simply click on your account name in the top right corner of PubMed to navigate to your account settings...on the “Filters & Icons” link (see image to the right). Once on the “Filters” page, select the category...

  30. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...science-themed submissions: string lights hung up in the shape of RNA, light-up neurons on the lab door, and...

  31. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... 2016, with the goal of providing easy access to high quality, commonly used viral vectors. Of course,...course, many scientists also need easy access to high quality, not so commonly used viral vectors. That’s where...

  32. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...aggregation of this information can provide valuable insight to the most requested plasmid, the number of requested... repository (you can find the link in the upper right hand corner of the dashboard). Therefore, users ...

  33. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...Data Hub If you're trying to decide which AAV is right for your experiment, searching the Data Hub's curated...experiments, let us know ([email protected]) and we might just send you a new Addgene Data Hub t-shirt!    ...

  34. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ... we mention graphs such as the one shown to the right that illustrate a grad student's happiness v time...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

  36. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...that is and has been to publish in journals with a high “impact factor,” a measure that dates back to 1975...Non-Elite Journals” found that the proportion of highly cited articles appearing in lower-prestige journals...

  37. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ... there are thousands of posters being fueled by highly caffeinated grad students and postdocs, which accounts...exhibit booths, happy hour socials, and late, late night socials. As a newbie to the SfN conference, I have...

  38. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...West African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement...links to the relevant scientific literature.  -In light of the current Ebola crisis, Science and Science...

  40. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...under the "Resource Information" heading in the right column of the plasmid page. Check out the purple...can also use the "Search Table" box at the upper right of the table to search and filter the list of citing...
Showing: 601 - 640 of 838 results