...more funding and research opportunities are being provided to immunotherapy than ever before. Although different...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...(ADAO) are working toward such a ban, and the president of the organization, Linda Reinstein – whose husband...

...fluorescence can occur. Cre recombinase can be used alongside common, existing tools for fly research that use...turnover of Langerhans cells in the dermis and identify that Langerhans cells are replenished through ...The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations." Proceedings...

...results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived...strand: A mix of degenerate primers and the high-fidelity Phusion polymerase are used to introduce point...

...found the video radd-ically helpful!    More resources at Addgene Addgene's Antibody Guide What is an...

...Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI’s well-validated...on our website and blog, including our antibody guide, antibody protocols, and Antibodies 101 blog series...

...      And you’ll be able to find all published videos in our Quick Tips playlist:  Want your quick tips...be sharing our favorite parts of every quick tip video on our TikTok! Be sure to subscribe to our YouTube...

...your favorite name. We hope you submit your best ideas because the finalists will receive some newly designed...Learn more about Blugene's make-over Get a how-to guide on the new Addgene search engine ...

...Sorting” is there to help guide you through the process. If you’re confident in your application and protocols...

..., tools, and knowledge. Guest authors The Codon Guide to Synthetic BiologyAuthor: Niko McCartyAntibodies...globe find and share knowledge, resources, and new ideas - we're so grateful for your writing, your knowledge...

...Learn more about the Data Hub in this blog post. Alongside our efforts to facilitate the sharing of reagents...Learn more about the viral service Read our AAV guide ...

...experience we're sharing through blogs, eBooks, videos, guides, protocols, and of course, the friendly folks...

...primary investigator. Coming up with new project ideas, applying for grants, and mentoring. Chapter 5: ...Find Synthetic Biology Plasmids Browse our CRISPR Guide Pages ...

...informative and entertaining protocol and explainer videos for you to level up in the lab, and we’ve started... could be you after watching Addgene’s protocol videos. via GIPHY   If you’re also looking for that...

... from IPI Simon Moe, from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating...Controls Priyamvada Prathima, who wrote a helpful guide to understanding your flow data. Flow Cytometry...

...awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The Baker Lab ...like high resolution Ras sensors and crosslinked peptides proposed as a scaffold for a new generation of...

...share validation and experimental data to help you decide if the antibody is a good fit for your experiment...improving antibody quality throughout research, consider checking out the Only Good Antibodies community...

...you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically...Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great Tools for Gene...

...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ... a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org Sign...

... their entire floor, and put together a festive video to showcase it all!           View this post...symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab with virus snowflakes from @CVRHutchinson...

...the diverse needs of our community and support a wide range of experimental designs. Here are nine great... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers...

... Addgene has been working with Seeding Labs to provide plasmid grants to researchers in developing countries...We are excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene...

...free educational resources: Our eBooks, videos, science guides, and protocols remain freely accessible...

...Benoit Giquel. Traveling from our offices just outside of London, it was a short journey for Benoit to...production. Chapter 5: The future of Synthetic Biology. Wide adoption of synthetic biology techniques in biology...

...commitment to top notch quality service and support.  Alongside our material offerings, in 2022 we also rolled...website saw 2.2 million visitors from 231 countries. Guides and protocols were our most popular content, while...

...search bar will change to an easy-to-use depositing guide, with links to our most helpful depositing articles...choices informed by Web Content Accessibility Guidelines (WCAG).  But don’t take our word for it — check...

...opportunities for career development and networking. Aside from the importance of the topics to be discussed...academic administrators who are finding ways to provide new types of career preparedness training for their...

...quoted as saying when the news first broke. "Considering that many human diseases are caused by genetic... and mainstream the news on CRISPR has gotten, consider these headlines in The New York Times and Forbes...

...design and difficult to multiplex. The Covert Lab provides an alternative tool with their Kinase Translocation...nuclear translocation rates are another variable to consider when designing KTR experiments. Third, KTRs are...

... who started the BALSA group, a nonprofit that provides short-term consulting services. Initiative and...?" Form Your Own Peer Mentoring Group: A How-To Guide for Scientists Download our eBook: Mentoring for...

... life sciences. In this post, we'll give you an inside look at the Top 10 plasmid technologies distributed...Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid. A variety of...

...this system was optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...Spinochordodes tellinii‘s larvae makes its home inside grasshoppers and crickets. When it’s ready to transform...

...Science Careers’ myIDP tool. Using myIDP, you can identify suitable careers based on your current interests...anxiety of graduate and postdoc training. On the other side, the PI has an opportunity to check in with lab ...

... own unique protein fusions. This collection is ideal for tandem affinity purification of protein complexes... describe a modular collection of plasmids for a wide variety of genome modifications in S. Pombe, including...

...antagonist. This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine...Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ...

...respectively, making it ideal for large gene transfer. The M24 promoter provides constitutive, high-level...

...dramatically increase the chances of being seriously considered for an open position.  Check out Joanne's Reddit...network your way to a hiring manager. Learn to confidently describe your strengths in your resume and don't...

...Boston University last week.  This fantastic city-wide effort was led by two amazing postdocs, Kristin ...about academia and alternate training needs to be provided to take advantage of all the possible science ...

...way to share their plasmids. Now I am on the other side of the force and as an Addgenie I will do all my...scientific discovery or invention, and sharing those ideas with others. This love of interacting with people...

...from the labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that...

...occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method...

...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running on a time crunch...

...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

...the SPARC system, attP sites are located on either side of a stop cassette, upstream of an effector gene...Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Nature Methods...

...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

...well-known reward chemical and neurotransmitter. But aside from DA’s famous role as the “happy hormone,” it...Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research ...

...Intelligence or to hone delegation skills, for example. So aside from a focus on removing obstacles, what makes a... (this actually varies, a manager does best to identify the work style of each team member) Need challenging...

...primary antibodies can be expensive and may not provide enough signal to visualize low-abundance targets...your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your primary ...

...aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition...

...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different...