...to monitoring localization; using FP timers, researchers can look at a single image of a cell and understand... production of the initial FP timer in 2000, researchers have realized how problematic aggregation-prone...expression. In a developing embryo, for example, a researcher could determine whether or not newly synthesized...synthetic biology, FP timers might be able to tell researchers whether old or new cells are better at producing...

...luminescence have been widely used in biological research for a long time, chromoproteins are just now being...history of fluorescent proteins in molecular biology research, chromoproteins have a shorter history. In 1987... can be used, why not add some color into your research? If you have a favorite chromoprotein, either ...Addgene.org Find fluorescent proteins for your research Read our Molecular Biology Reference Find plasmids...

...fluorescent tool with magnets would enable new research and applications that don’t yet exist, but it ... this was even possible. There is a variety of research on whether and how organisms respond to magnetic... non-biological conditions. So, they decided to search for a magnetic response among other proteins that...some hypotheses. They are excited to see other researchers from a range of fields, like quantum chemistry...

...with any scientific research, choice of a technique often comes down to the researcher’s familiarity with...the nature of the experiment, it is up to the researcher to determine which method is most appropriate...stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus...

... doors for scientific research - how will you use these systems in your research? Cary Valley contributed...developed and potential ways you can use it in your research. RNA editing advantages RNA editing has multiple... forward to the expanded use of RNA editing in research and its potential therapeutic uses We are also...

...they research, how they conduct their research, and in some cases, if they conduct their research. Even...inherent brokenness of scientific publishing and research funding. I’ve been aware of these problems since...

... Resources on Addgene.org Search for AAV Vectors to use with your research. Visit Our AAV Guide Page ...a key challenge of using AAVs for neuroscience research is the lack of a method for genetically manipulating... new AAVs can serve as tools for basic science research (neuronal tracing and morphologic studies), as...

...experiments that work anywhere in the genome. Aneesh's research interests include proteomics, machine learning...Taking a nerdy perspective, you might denote your search as follows: (my_house + coffee) - starbucks See...Data. Quilt is a collaborative database. Aneesh's research interests span machine learning, proteomics, and... on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find Validated gRNAs...

...understanding the brain’s function is knowing its architecture, in particular the connections between different...to their advantageous characteristics for brain research and their ability to introduce genetic material...fluorescent proteins (red, green, blue). If a researcher injects a cocktail of these different AAV vectors...Atlas website. A similar approach was used by researchers from the Janelia Campus for their MouseLight ...University of California, Berkeley, the Janelia Research Campus also developed a new AAV variant, rAAV2...

...in neuronal circuits or synaptic remodeling, researchers combine the Cre and FLP recombinases to control...combination of Cre, FLP, and inducible systems gives a researcher exquisite control over expression of multiple...Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how...-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids...

...the College of Life Sciences that same year. Her research is focused on the escape mechanism of genome editing...Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-encoded...professor in Synthetic Biology in 2006. The Yang lab's research is focused on developing microbial genome editing...Sciences in September 2019 as a Ph.D. candidate. Her research focuses mainly on the development and application...

...and enrich base editing activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124...cleavage by Cas9 in living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332...enrichment (TREE) in human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713...the CRISPR guide Find CRISPR plasmids for your research ...

...users, we just released a major redesign of how search works on our site. For Addgenies, development includes...with our software. When this happens, we need to research and hopefully find a comparable library if it’...our users focus on the fun stuff, accelerating research and discovery. We work with transparency, gladly...the Addgene blog Learn about what went into our search engine update Find all Addgene news Resources ...

...proteins included in different CGBE tools allow the researcher to direct the repair of the abasic site. But ... originally being developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency...transversion editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing...existing CGBEs. This distinct approach means researchers can now select the best tool for their application...

...signal at a point on the axis determined by the researcher, based on their controls. You can usually tell...intimated if you’re struggling to understand how the researchers decided to place their gates on the plots! Flow...gating strategy, you can start to ask why the researchers made the decisions they did in their experiments...vitro chondrocytic differentiation: A stem cell research initiative at the King Abdulaziz University, Kingdom...

...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...it’s time to use them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing...RNA-guided endonucleases and nickases." Genome research 24.1 (2014): 132-141. PubMed PMID: 24253446. PubMed...

... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... variety of ways to manage my time. As a busy researcher, you’ve probably already started coming up with...hope you’ll find this post useful. After a bit of research and thinking about my own processes, I’ve broken...

... contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding number of ways...attending physician. He performs post-doctoral research in the lab of Matthew Waldor at Harvard. Now that...sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID: 24234441. PubMed... unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID: 25800749. ...

...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if your...this file over the years and a talk about your research or work will write itself when the time comes....ask about how many people use plasmids in their research and then see how many of them have requested from...

...through the use of invasive methods, limiting researchers to accessible, spatially constrained regions.... it’s always helpful to start with a literature search to see what others have done, you’ll need to experimentally...precursor. Considering systemic capsids for your research? While systemic capsids mostly function like your...depicting the phylogenetic relationship of common research species. Among these species, Old World Monkeys...to the nonhuman primate nervous system. Current Research in Neurobiology, 4, 100086. https://doi.org/10.1016... central nervous system. Nature Cardiovascular Research, 1(4), Article 4. https://doi.org/10.1038/s44161...