We narrowed to 582 results for: BASE;

...and publish a human-designed protein that was not based on any existing protein structures, called Top7....

...event, and assigns each event to a channel number based on intensity. Higher intensity events are assigned...to the protein in its native state.  Antibodies-based flow cytometry can use both direct and indirect ... background information for setting up antibody based flow cytometry experiments.       References and...

...is vital for the accurate interpretation of FRET-based experiments. A popular combination is Alexa594, ...dyes, expanding the capabilities of fluorescence-based techniques in biotechnology. Recent examples of ...2016). Development of a quantitative fluorescence-based ligand-binding assay. Scientific Reports, 6, 25769...

...questions, which are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent...different city, you can still develop a successful FRET-based assay since energy transfer only occurs when donor...10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic...

...multiple FP(11) fragments, even from a different base FP (for example, EBFP2(1–10) can be used with GFP... plasmids from the associated paper:   Color Base FP Description Article (Link to Plasmids) PI ...

...point where the hydrogen bonds break between the base pairs. This results in the separation of the two...using PCR. qPCR requires the addition of a probe based fluorescent dye that intercalates with any dsDNA...

... scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the world to combat...deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent...

...but their initial results show promise for CRISPR-based gene activation. dCas9-CBP In this method, dCas9...2013) RNA-guided gene activation by CRISPR-Cas9–based transcription factors. Nat Methods 10:973–976 . ...

...quickly and inexpensively generate a potent Cas9-based transcriptional regulator without making any functional...date his group has develop several popular CRISPR-based tools for altering DNA sequence or regulating gene...

... John Doench et al. shows that mutating a single base pair may not be enough to prevent re-binding. Lastly...region in a UTR, CrispyCrunch flips the two important base pairs of the PAM (NGG -> NCC, CCN -> GGN). This ... much as possible, it uses standard tools and databases, including primer3, biopython, bwa, samtools, ...

... electroporations. The specifics of this can be based on the manufacturer’s protocol for transformation...transformation efficiency, or more specifically calculated based on the size of and weight of your library plasmids...

... different categories based on function: knockout (KO), activate, inhibit, base edit, and other. The first...

...available filtering options now change dynamically based on the type of material and specific search terms...curated pages categorized by both functionally (e.g. Base Editing) and by target organism. Clicking through...

... question to ask is how the efficiency of PITCh-based genomic insertion would compare to CRISPR sticky-end...Mathieu, et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human...

...and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed to detect...separates mixtures of cells into distinct populations based on fluorescent signal. Thus, FACS can be used to...

...antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX multiplex tissue imaging Data repository...

...Novartis, a multinational pharmaceutical company based in Switzerland. After completing her PhD in Chemistry...

...genome and bind target DNA sequences using RNA-DNA base pairing. Separately, TnsABC forms a transposase ...tools such as Cas9-recombineering, CRISPRa/i, and base editing. Leveraging a unique marriage between distinct...

...demand model with weekend and holiday effects. Based on these observations, we considered the following...enable next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four...

...consistent development and improvement of CRISPR-based technologies over the past decade. CRISPR screens... fact supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large...