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We narrowed to 599 results for: ELP

Showing: 301 - 320 of 599 results

  1. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...density. In some cases, nanobodies have been found to help improve label density (Mikhaylova, et al. 2015)....approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal...

  2. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...fragments You might be wondering, “How is milk going to help me extract DNA from agarose gel?!”   The DNA gel... it solubilizes both DNA and agarose, and 2) it helps DNA stick to the glass. After binding DNA, the glass...

  3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...genome-wide gain of function screens. These screens can help reveal the function of lowly expressed genes or ...with two MS2 RNA aptamers, and the  MS2-P65-HSF1 helper protein (Konermann et al., 2015). Root and Doench...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy...and colleagues at MEEI co-founded a non-profit to help push forward candidate therapies for these diseases...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...improvements to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as ...for the modification in the assay host strain can help. In order to reduce the chance that the configuration...

  6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...activities because I can contribute by, for example, helping to improve the papers submitted to journals. In...Japan, which is very disappointing. I hope I can help change the situation in the future. Addgene: What...

  7. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...RepZ to drive its own replication. Like RepA, RepZ helps recruit host initiation proteins to the plasmid ...Plasmids 101 Posts Get practical molecular biology help with our Molecular Biology Reference Pages   ...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ... lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using...stable and can withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...stuck trying to write one, hopefully this post will help you get things moving - remember you're becoming...Year) in the document while writing, because it helps you later on to remember where you read particular...

  11. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...For us, transient leaf transformations are a great help in screening candidate genes and speeding up research...transformations, but we hope that these tips might help to get you started. Once you have established a ...

  12. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...reliable results from CRISPR edit data requires the help of robust software tools. As a consequence, a critical...detected edit types in the sample. The visualization helps the researcher see which of the multiplexed sgRNA...

  13. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gene modified mouse lines. Hopefully this post will help get your in vivo research up and running as soon...Resources on Addgene.org Find Software Tools to Help Design You gRNA Find CRISPR Plasmids for Use in ...

  14. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ... that receive different types of stimulation may help to refine and optimize your stimulation parameters...Happy experimenting and hopefully optogenetics will help illuminate (is that pun worn out yet?) your research...

  15. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...are a few simple troubleshooting measures that can help get you back on track: Compatibility: Double-check...inducer or adding an affinity tag such as GST may help with solubility issues. We want to hear about your...

  16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...SlideShare, Figshare and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload the...their data. Likewise, we want to contribute to helping others understand ours. Improving access to data...

  18. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... your gel. At their most basic, loading controls help you rule out errors like uneven pipetting or irregular...necessary to demonstrate a POI’s absence. It also helps confirm that lanes are loaded evenly. Created with...

  19. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... probes, we decided to write this quick guide to help researchers choose the probe for their needs. You...assays with other FPs.   We hope that this post helps you in getting to know the state-of-the-art NIR ...

  20. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... phage display library with plate-bound antigen helps select for high affinity scFvs. Bound phage are ...expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm (Sandomenico...
Showing: 301 - 320 of 599 results