... use of several Cre transgenic mice targeting different brain areas enabled the drawing of a detailed ...the genOway press release for more information. References Abdallah, Khaled, et al. "Adeno-associated virus... delivery of Cre recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed .... PubMed Central PMCID: PMC3979449. Fenno, Lief, Ofer Yizhar, and Karl Deisseroth. "The development and...and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical reality." Neuron...comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical and biophysical ... Addgene website Visit our molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids...

...saw that these AAV particles come in a slightly different shape. These AAV particles are often found with...able to infect more cells (as demonstrated in different cellular environments such as retina or glioblastoma...purification protocols for isolating exo-AAVs rely on differential centrifugation steps to separate cell culture...Exo-AAVs have improved transduction profiles in different AAV serotypes both in vitro and in vivo when compared...to inactivation through anti-AAV antibodies. References György, Bence, et al. "Naturally enveloped AAV...PMC3345986. Meliani, Amine, et al. "Enhanced liver gene transfer and evasion of preexisting humoral immunity with...

...efficacy of different materials so that we could have discussions about the pros and cons of different fabrics...about the difference between airborne and aerosolised droplet transmission and how different fabrics and...sexual and reproductive health.    References and Resources References Drummond, C., & Fischhoff, B. (2017...

... is it different from a CV? Get familiar with the general format of a resume and the different presentation...Wondering how you can develop more transferable skills? See Developing Transferable Skills During Science Training...advantage over other candidates. Write about your transferable skills Some of the skills you developed might...have presented in some public lectures? These transferable skills could be meaningful for your potential...

...cancer due to its involved in cell proliferation and differentiation.  Since Ras is a membrane localized...gene expression changes lead to cell proliferation and differentiation. The lab confirmed this OptoNB-SOS...OptoNBs that bind cell signaling receptors would offer more levers to pull to control signaling pathways...for more uses both inside and outside cells. References Gil, Agnieszka A., et al. "Optogenetic control...

...work to. Before submitting your paper to your preferred  journal, it is important to check the journal...reviewer’s name and affiliations, and the entire referee report are permanently attached to the published...authors and readers are able to comment on the referee reports. If the reviewers suggest that the manuscript...of the article, while the first version and the referee reports remain available online. Open-peer reviewing...reviewing is beneficial for both the referees (as they get credit for their time and effort) and the scientific...prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread your manuscript...

...transition from a clump of cells to a fully differentiated body. In contrast, these dynamic processes ...distinguishing cell populations that have been tagged with different fluorescent proteins or dyes. 3. Two-photon microscopy...cells. For example, by harvesting lymph nodes at different times after antigen challenge, scientists were... see movement and interaction between several different immune cells. These methods allowed immunologists... like this is a mosaic of cells labelled with different fluorescent proteins. Using one iteration of this...understanding of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging...17145958. PubMed Central PMCID: PMC2118178. 6. Schaeffer, Marie, et al. "Dynamic imaging of T cell-parasite...

...poised for more offerings. The viral vector team is publishing research, hosting conferences, and are expanding...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received...step to maximize the services and features we can offer online for Addgenies and to serve external customers... a lot of successful candidates from internal referrals. Our long reaching reputation helps us hire great...practices and benefits and these are making a difference. We serve scientists around the globe and our...

...sources. Mode of communication Different modes of communication take up different amounts of mental bandwidth...definition of cognitive load states: “cognitive load refers to the used amount of working memory resources....with a specific topic; extraneous cognitive load refers to the way information or tasks are presented to... to a learner; and germane cognitive load refers to the work put into creating a permanent store of knowledge...clearly as you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

... hand and use them to easily test different antibodies or different signaling molecules. Many labs find...antibodies to bind to a primary antibody without interfering with the hypervariable region, where an antibody...are readily available conjugated to a number of different signaling molecules. With the direct approach,...possible, or ensure that the primary antibodies are different species and/or isotypes. For highly multiplexed...generated in goat against rabbit immunoglobulins. References and Resources More resources on Addgene’s blog...

...a property of the fluorescent protein. This can differ between fluorescent proteins in the same spectral...dependence on excitation power is important since different fluorescence imaging strategies use widely varying..., TIRF or spinning disk confocal use entirely different excitation power regimes. Therefore, it is unpredictable...the best way to compare the photostability of different fluorescent proteins? Measuring photostability...excitation power. By repeating these measurements for different fluorescent proteins and by comparing how the ...measurements reported in the literature are performed in different ways. We describe a number of issues with some...

...created a generalizable tag-based strategy, which we refer to as the degradation tag (dTAG) system, to co-opt...selectivity, broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG...expression of these cereblon neo-substrates varies in different contexts (for example, IZKF1 and IZKF3 are cell-type...technology. Follow him on twitter @behnamnabet.   References Nabet et al. The dTAG system for immediate and... 14(5): 431-441. PubMed PMID: 29581585. This reference contains the full description of the dTAG system... Huang et al. MELK is not necessary for the proliferation of basal-like breast cancer cells. eLife. September...

... These each have different goals, formats, and processes, and they provide different amounts of access...research during his PhD studies.  References and Additional Resources References ASAPbio, Preprint Resource ...like publishing a research article, presenting a conference poster or talk, or submitting patent or grant...Going to present some unpublished results at a conference? Post your working manuscript as a preprint too...

...scientific manuscript will require numerous other references to scientific literature to substantiate the ...literature related to the topic. Finding reliable references and sorting out what they mean is no small task...Did they perform all of the appropriate controls? Refer back to the methods section for finer points of ...inspiration and accountability. There are a few different ways to automate the process as well. Sign up ...with scribbled notations in the margins. If you prefer, printing and hand-writing notes is still possible... Check out Papers and Mendeley – these programs offer more options for searching and sorting your own ...

...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely used...distinct complexes may explain how more than 300 different coactivator proteins individually contribute to...patterns of nuclear receptors across multiple different tissues in normal and disease states, their circadian...

...plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of dCas9 expression...system developed to track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a ...cell surface. When the cells interact, GFP is transferred to the receiver cell, and the receiver cell turns.../genetics/iyab006       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith Joung...

...some of Cas9’s shortcomings. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both...pattern opens up the possibility of directional gene transfer, analogous to traditional restriction enzyme cloning... cloning. Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells...immunogenicity and range of serotypes allowing preferential infection of certain tissues. However, these...Cpf1 fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim...

... one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG). There...customizability. It has a large deck that can house many different types of devices. As long as a device fits on ... - one pod can pick up 96 tips for easy sample transfer between 96 well plates. The second pod can pick...out your samples. This can be useful if you’re transferring samples from 96 wells plates onto agar q-trays...follows all the same steps and uses the similar buffers as would be used for a mini prep, except it does...video library Check out our molecular biology reference pages ...

...chromoprotein and how is it different from fluorescent proteins? Before going into the difference between chromoproteins...research Read our Molecular Biology Reference Find plasmids for FRET References Blanquet RS, Phelan MA (1987...imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure 4). They created... Jennifer Tsang...

...rely on two-step hierarchical approaches using different Type IIS restriction enzyme specificities at each...Factors such as enzyme efficiency, stability, and buffer compatibility have placed practical limits on single...Assembly test systems Our research focused on three different levels of assembly: High efficiency and accurate...biotechnological cloning efforts for over 50 years, prefers ligation of Watson-Crick base pair substrates, ...products to market, New England Biolabs, Inc.       References 1. Vladimir, Potapov, et al. "Optimization of...cloning protocols Check out our molecular biology reference pages ...