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We narrowed to 712 results for: Met

Showing: 301 - 320 of 712 results

  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...experimental parameters (e.g. excitation light intensity, pH, or temperature). pH Stability: This parameter is ...Photostability can be affected by experimental parameters (e.g. excitation light intensity, pH or temperature... short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for most FPs it is a few seconds ...

  2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns out over time...signals. Let's say I wanted to detect every primary metabolite in a cell. The only way to do that at scale right...can now forward engineer a 10-gene system to do something. We know from our testing of these parts that ...transcription and translation initiation." Nature Methods. 10, 354-360 (10 March 2013). ...

  3. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...The One Method to Rule Them All, consider the following four questions to figure out which method(s) work...proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications... lot of trouble in the long run.  Selecting the method Once you’ve answered the above questions, you can...

  4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...general method to improve fluorophores for live-cell and single-molecule microscopy." Nature methods 12.3...cell biologists talk to chemists? One reason is something called “the photon budget”: each sample has a ...self-labeling tag systems (Figure 1b) are perhaps the best method for live cell labeling and the easiest switch from...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...floral drip or dip transformation method. The general steps for this method include: Cloning and transforming...Harrison, S. J. et al. (2006) ‘A rapid and robust method of identifying transformed Arabidopsis thaliana...seedlings following floral dip transformation’, Plant Methods. BioMed Central, 2(1), p. 19. doi: 10.1186/1746...

  6. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... or even a synthesized gene. PCR is the primary method to produce the linear fragments, as desired modifications...C for 15 mins). This enzyme specifically cuts methylated DNA, so it will selectively destroy the template...Jones, Douglas H., and Bruce H. Howard. "A rapid method for recombination and site-specific mutagenesis...5 factors to consider before choosing a cloning method Check out all of our blog posts on plasmid cloning...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...potential to become resistant to? CRISPR may provide a method for doing just that. While challenges remain in...resistance will be slow. CRISPR may be just the method needed to jumpstart the fight against antibiotic...certain bacteria, just like the CRISPR targeting methods. Phage cocktails could be used to target multiple...Additional Resources on the Addgene Blog CRISPR Methods for Bacterial Genome Engineering Treating Muscular...

  8. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...immunoprecipitation (locus-specific ChIP). These methods consist of insertional chromatin immunoprecipitation...to describe?  Fujii: I believe that "Biology is Method", i.e. new technologies often lead to big discoveries...oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification of specific DNA sequences... they don't stop, they may be able to achieve something that may change the rules of the game. In this...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...perturbing circuits together with the development of methodologies to deliver genes to the CNS have recently made...use epitope tags to monitor single molecules - something researchers often want to do at neuronal synapses...microscopy. Sample preparation for these techniques sometimes makes it more difficult to detect protein tags...probes for light and electron microscopy." Nature methods 12.6 (2015): 568. PubMed PMID: 25915120. PubMed...

  10. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... for your brainstorm session is to start with something completely unrelated to the issue and consider...out-of-the-box concept and pare it down until you have something tangible and applicable to your situation.   Thinking... We become so fixated with the idea of making something the best it can be that it seems impossible to...stuck on a problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing...

  11. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...years, but it usually ranges from 20-50 years and sometimes longer. There is no known safe level of exposure...has caught it has usually started to spread (metastasize) and turned into Stage 3 or Stage 4 cancer. These...chemotherapy drugs used to treat mesothelioma – pemetrexed and cisplatin –because it cuts off the blood ...in the world. The development of new diagnostic methods and treatments such as these has brought a lot ...

  12. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...pair palindromic repeats separated by an 8 bp asymmetric core. Recombination between these sites can lead...discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is ... recombinase activity (Logie and Stewart 1995; Metzger et al. 1995). Visualizing single neurons in the...PMID: 7597057. PubMed Central PMCID: PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “...

  13. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ..., but for many of us, scientist is something we are, not something we do. We have to find a way to keep...try to keep discussions on-topic and, at least sometimes, civil. r/Science in particular hosts a variety...scientists in training is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s...

  14. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...understood, but some possible reasons include: Metabolic pressures - it takes cellular resources to produce...optimized for a particular organism, this will sometimes (but not always) be noted in in the “mutation”...of your protein of interest. Regardless of which method you choose to overcome the problems surrounding...surrounding codon choice, you should have some method to make sure the proteins you produce function properly. ...

  15. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels... storage buffers used, and other experimental parameters. For example, while freeze thaw is generally ...H.G. “Detection of mycoplasma contaminations.” Methods in Molecular Biology 946 (2013). 1-13. Pubmed PMID...PubMed Central PMCID: PMC3456965. 11. Zhang B., Metharom P., Jullie H., Ellem K.A., Cleghorn G., West M.J...

  16. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ... restriction/methylation system. This means the strain can neither restrict nor methylate DNA. dcm: Similarly...Similarly, strains with this mutation are unable to methylate cytosine within a particular sequence. Browse...of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11...

  17. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...approach combines the overlapping and trans-splicing methods. The AAV encoding part A of the transgene contains...of the transgene have been seen with the hybrid method. While these fragments are likely non-functional...Frischmeyer et al., 2002). How to pick a split AAV method While the hybrid dual vector system has a slight...Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140...

  18. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...cassette. This leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes...leave any undesired scars in the chromosome. These methods roughly fall into two categories: those that rely... a sucrase that converts sucrose into a toxic metabolite. Cells that have undergone a double crossover...identify double crossovers. This can be an efficient method to generate precise, unmarked mutants, but it frequently...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...helpful. I set out to make something practical and accessible. If there's something you come across that is...The second question is whether there is chalk. Sometimes. It can also be a slide deck, markers, etc. Chalk... What is the big question? This should be something that anyone could relate to, that people would...

  20. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, R...concentration changes in response to experimental parameters. This is a job for a semi-quantitative western...not especially linear. In panel C, two different methods of quantifying total protein are compared. While...
Showing: 301 - 320 of 712 results