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We narrowed to 849 results for: NTS

Showing: 301 - 320 of 849 results

  1. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...for this project, what would be your critical experiments and why? What techniques and skills do you ...another in a way not done before in your field?” He points out to “not be shackled by your own subject - maybe...applied to fungal biology.” Connie Cepko advises students to consider their goal of PhD training when selecting...” Professor George Church encourages graduate students and postdocs to follow their passions and dive...Research projects are designed to train graduate students and postdocs to think analytically and critically...elucidate which project is right for you.   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College...

  2. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    Published
    Oct. 10, 2019, 12:41 p.m.
    ...as antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et...different parts together. By combining two optimized elements of an antibody probe, scientists can now more ...the widely used HA epitope in diverse cellular environments. They called this probe the HA frankenbody (... HA (smHA) which contains a 10xHA tag. These experiments demonstrated that the HA frankenbody can be used...tool-kit at Addgene to get started with your experiments! What else can you do with the HA frankenbody...

  3. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...highlights TTOs that accelerate science by processing agreements quickly! If your TTO receives a Golden Stopwatch...agreement. Below, you will find some easy talking points for broaching this subject with your institution...professionals: Describe to your TTO how unprocessed agreements slow down your research; provide specific examples...against one’s own record Mention Addgene Master Agreements. By default, your TTO must sign an agreement ...respective Master Reception and Master Deposit Agreements, your TTO signs each agreement just once, covering...the processing time of request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest...

  4. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...extranuclear agents of heredity were thought of as everything from parasites, to symbionts, to genes and...Wollman and became the widely adopted name for these elements. At the time, the use of episome seemed appropriate...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved... vectors that could be used in these cloning experiments. Although plasmids started as a somewhat niche...that can be diversely applied to many different experiments. Addgene was founded in order to store, QC, curate...

  5. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...funding and space in academic research labs has more students looking for other options. While graduate programs...academic career development, it's often left to students to identify and build the necessary skills for...research from a broader viewpoint may expose which experiments are crucial in demonstrating the true innovation...entering the workforce increases, EAs allow graduate students and postdocs an opportunity to tailor their career...interests, and skills? Curious to know where graduate students and postdocs are currently getting hired? Let ...more about next. Leave your suggestions in the comments section.  ...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should be used to clean...from workspaces. Speed: AAV transduction experiments are relatively quick. We have found that expression...benefits make AAVs useful for a wide variety of experiments, care still needs to be taken when selecting ...complexes are so robustly expressed that anatomical assessments can be easily performed too. While I implied ...transport of AAVs is a potential problem in many experiments, a group of researchers has identified this feature...used to treat disease in humans and additional improvements are being made to make them even more useful...Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources on Addgene.org Find AAV...

  7. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ... none of the participants had preconceived opinions about “adeolitis.” The participants in my study were...used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers... affirm or challenge their beliefs. I asked participants to read short abstracts comparing the mortality...ideas about the controversial medical issues. Participants gave significantly more favourable ratings to...texts were otherwise identical. Likewise, when participants read an abstract for a study with findings that...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...new NEB kit includes a step that both fragments the DNA and prevents it from forming concatemers later. ...services and expertise without breaking their bank accounts. When I heard about how LabCentral aims to enable...must first fragment a DNA sample into many small components. Without proper processing, these small DNA strands...produced high yields of quality libraries from small amounts of DNA i.e 100 pg. At the start of the workshop...itself. This was great because it forced workshop participants from different biotech companies to interact...

  9. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...known as a *library*. Parallel sequencing of the fragments within a library allows researchers to reconstruct...together to create a single library containing fragments carrying sample-specific barcodes. Rather than...is an author of 20+ scientific publications and patents.   ...

  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ... important to know what kind of reagents you work with in experiments. Hence, I’m including this MSDS ...do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made by New England...AMINO can be beneficial for new researchers or students beginning to learn about biochemistry. The least...have a favorite lab app, please share it in the comments section below! Many thanks to our guest blogger...Pseudomonas aeruginosa. Follow him on twitter @100percentSDS.     Additional Resources on the Addgene Blog...

  11. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...collaboration - a sense of dialogue and community among participants, clarification - new and useful information,...them manageable. At Addgene, teams can add as many points as they like to the written agenda, but each only...and Accountability Agendas: Send around talking points for the meeting a day in advance. This gives everyone....  During the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen...for talking, not reading: Send around relevant documents, like proposals to review or project plans, before...send around a Google doc where people can make comments. At meetings, we just focus on the parts where...

  12. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...reported brightness measurements. Here, EGFP outperforms the improved folding variants, presumably due to.... A common requirement for live cell imaging experiments is the ability to follow multiple fluorescently... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...channels used for more abundant proteins or marking compartments. I hope this sheds some light on multicolor ...

  13. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...substrates can permit measurements of luminescence from live cells. Luciferase variants with incompatible ...proteins offer different kinetics, substrate requirements, and photon emission wavelengths. In addition...analysis to investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated...binding interaction brings the two luciferase components together. A similar approach can test protein-protein... substrates can also allow multiplexing with experiments that supply both substrates and measure luciferase...

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...response elements (TRE) in a promoter. Tetracycline and its derivatives serve as inducing agents to allow...inactive because a bound repressor protein actively prevents transcription. Once an inducer binds the repressor...turned ON. Types of inducible promoters Chemical agents, temperature, and light are all examples of factors...HSF-1), which subsequently binds to heat shock elements in the promoter, thereby activating transcription...various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters and there is very...

  15. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    .....we’re excited to help scientists share their reagents with the scientific community.   (click to enlarge...it ideal for CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through... Zhang lab has shared over 40,000 CRISPR-Cas9 components over the years on their own or through Addgene...used as a negative control for mammalian RNAi experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC...Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse transcription, and integration...our depositors and requestors for making their reagents available and for supporting scientific sharing...

  16. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ... to use the Gal4/UAS system, but the available reagents do not match your needs. If YFG is thrillingly...generate a transgenic fly There are a few important points to consider when choosing the vector you’d like...comparisons between lines expressing different variants of YFG because the transgenes are found in the...won’t be positioning effects). When designing experiments where you want to express multiple transgenes...chromosome your docking site is located). This amounts to a small difference of two or three weeks. 3....for. Making your transgenic fly and downstream experiments Once your gene of interest is cloned into a plasmid...

  17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...diseases. They do that by studying patients to uncover new gene variants associated with conditions of interest...interest, then studying those variants in model systems: either human cells or mice. In a recent issue...implicated in disease or, in cases where particular variants or mutations have been reported in people, we .... You could use the kit to insert all of those variants into stem cells with the same genetic background...make induced pluripotent stem (iPS) cells from patients and try to compare those lines, they may all have...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...used together in the same experiment because the reagents used to detect the presence of an antibody depends...applications for antibodies You can use antibodies for experiments in a variety of fields including neuroscience...lysates, whole cells, or tissue samples. Many experiments that use antibodies use a primary antibody, which...buffer. Then, the purified protein can be used in experiments to compare the relative amount of protein present...such as Western blots or ELISAs, or for other experiments to study protein-protein interactions. Flow cytometry...2016) Aptamers, antibody scFv, and antibody Fab’ fragments: An overview and comparison of three of the most...most versatile biosensor biorecognition elements. Biosensors and Bioelectronics 85:32–45 . https://doi.org...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...experiment work? What are the various tools and components necessary to run it? And, most importantly, what...optogenetics: 1) electrophysiology and 2) behavioral experiments. I’m not an electrophysiologist so I cannot speak...any optogenetic experiment. There are numerous variants for the different types of opsins available and...these reviews for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn...learn about the many different opsin variants and how they can be used is to consult the many primary research...in which you monitor expression at various time points (e.g. every 1-2 weeks over 8 weeks) in order to...science considerations for your optogenetics experiments. Stay tuned!  Many thanks to our guest blogger...

  20. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...ChRger. In both experiments, neurons expressing ChRgers produced stronger photocurrents at low light intensity...invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are commonly ...properties best suited for less-invasive optogenetics experiments. The sequences in this library were designed ...in vivo minimally invasive optogenetics. Both experiments delivered ChRgers by intravenous injection of...the light was turned off.  Together these two experiments determined that ChRger2’s improved light sensitivity...
Showing: 301 - 320 of 849 results