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We narrowed to 745 results for: Nes

Showing: 301 - 320 of 745 results

  1. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...assignment to discuss goals, expected outcomes, timelines and deadlines. Agree on a way to review project progress...identify constraints and boundaries. Where are the lines of authority, responsibility and accountability?...try "Why Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation tips...

  2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...cloning uses short sticky ends to insert genes into plasmid backbones, base pairing between these ~4 base ... different types of PCR-generated inserts. By harnessing the power of DNA repair, you can assemble multiple...2024.  References and Resources Further reading Harnessing homologous recombination in vitro to generate...

  3. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...and funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity...variable actually makes a big difference for the robustness of the findings.   How do we make research more...code, and other materials. The use of reporting guidelines can also help remind us to include all of the...

  4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...PAM sequence that the Cas nuclease recognizes determines site accessibility. Cas9s with a broader PAM ...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...learn new tasks and beat humans at the ancient Chinese game Go). In addition, there was an entire story-telling...neuroscientists are using techniques from across different disciplines to better understand neurobiology and human cognition... provide a highly versatile means of delivering genes to many different cell types with minimal immunological...effects. AAVs are used in experiments targeting genes to specific cell types in the brain, mapping neuronal...

  6. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...observe a phenotype, the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are commonly... al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms tRNA-gRNA and tRNA-gRNA-tRNA...constructed PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs...knockout, or multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also...

  7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion-pump encoding genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction...fluorescent protein. This GEVI features increased brightness and improved plasma membrane specificity. Stable...encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID...

  8. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et al., 2017). However, Acr inhibition...What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires...compatible approach to simultaneously clone two genes-of-interest (GOI) into two independent expression...

  9. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...definitely not worried. I have confidence that from Business Development to Shipping we will execute any new...thanks to evolving outreach tactics, increasing business development efforts, and our London team. We show...Atlantic. We’ve been in documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. ...dedication, flexibility, innovative thinking, and openness to team work.   Company culture Once in awhile...

  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Chen et al. successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the...of fluorescent puncta observed. By labeling two genes of a given chromosome simultaneously, they could...also monitor the spatial relationship of the two genes over time, at a range of distances from 2-75 Mbp...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...

  11. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...clinicians, researchers, and even farmers are harnessing these molecules for good. RNA:RNA dsRNA and ...exploited for antiviral purposes similar to that of vaccines. If you know the sequence of a virus, you could...dsRNA. Synthetic dsRNA Researchers have been harnessing the power of dsRNA for a long time, even before...complete. However, transcription of thousands of genes is constantly occurring throughout a single cell...

  12. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...Science Foundation, and Stanford University—that harnesses the creative power of sex and gender analysis ...sex of their cells. For one thing, cells and cell lines degenerate over time—and sex can be hard to determine...indicating the sex of the most commonly used cell lines appearing in American Journal of Physiology-Cell...Engineering, and Environment—a project aimed at harnessing the creative power of sex and gender analysis...

  13. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...directions of the project, Outlines a detailed scope of specific aims, milestones, possible setbacks, and...will address these limitations and lead to new milestones in the field. Research strategy The Research...proposals consist of 3 to 5 aims, with secondary milestones to meet along the way to achieving each aim. ...

  14. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...in your career path and still find comfort and happiness there. Read all of the Meet an Addgenies features...Fabienne Furt, Addgene’s Associate Director of Business Development, got her PhD in Plant Biochemistry...she joined an investment club and discovered the business side of science, she stuck with it. Lab Manager...people, and making them get outside of their comfort zones. I came to Addgene with a goal to learn nonprofit...

  15. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...that takes advantage of DNA sequencing to turn mAb clones into expression constructs to be conveniently modified...long-term solution to reproducibility and cost-effectiveness for broad and large quantity uses. We therefore...accuracy while screening. We also compare different clones of the same target to see if the results are the...primary antibody compared to negative control also determines the signal’s strength. Exposure time is the amount...

  16. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...spike protein can be used for the development of vaccines and diagnostics (Hsieh et al., 2020). As the virus...diagnostic enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase and Taq..., from Addgene to better understand which human genes are important for SARS-CoV-2 entry into human lung... information on these plasmids and a behind-the-scenes glimpse of their work, head over to their blog ...

  17. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...cells, one may have the unfortunate experience of witnessing…nothing. For some unclear reason, cells are not...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic...conditions for storage, transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603...receptor-independent virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. ...

  18. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... such as LTRs, gag, WPRE, antibiotic resistance-genes, or the transgene itself. Many researchers prefer...that target the common features of viral vector backbones; once validated these universal primers can be...popular approach utilizes the antibiotic resistance genes carried on transfer plasmids. Target cells are transduced... for predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed PMID: 15291957...

  19. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...H840. To ensure that the clones were capable of cleavage, they tested the clones against an NNN PAM library...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...variants recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition...

  20. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... do everything as I planned and I was missing deadlines - which puzzled me because I could do all these...basis! The silence and maybe even the slight awkwardness can spur people to speak up. Once aware of the...to be!  References Becoming the boss. Harvard Business Review, January 2007. Three Rules for Managing...  Are Good Managers Born or Made? UPenn Wharton Business School Blog Additional Resources on the Addgene...
Showing: 301 - 320 of 745 results