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We narrowed to 591 results for: SIM

Showing: 301 - 320 of 591 results
  1. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... tends to go something like this:   However, as simply as it seems laid out above, there are many places...potential programs and universities stressful or simply not feasible. Through the virtual systems created...
  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...Pooled lentiviral CRISPR libraries (referred to simply as “CRISPR libraries”) consist of a heterogeneous...
  3. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...
  4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully engineered...incredible hidden story here. The parts we are releasing simply plug into this existing economic activity. People...
  5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...
  6. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...
  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...
  8. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...non-scientist friends and family is Part Five, called simply “Bad Science.” Zimmer kicks off the section saying...provides several. In Part Three of the book, called simply “Old Science,” he describes the now infamous tale...
  9. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...
  10. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...
  11. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...doors. It would be a nightmare if we didn’t have a simple way to track all of our 40,000 samples available...  If a plate of samples is ever dropped, we can simply scan the tubes and know what is in each one. Even...
  12. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...to two fold for the final titer value, which is simply due to the noise of the assay. In other words, ...). However, because purified AAV preps contain similar and low levels of background protein and chemical...
  13. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... in different locations all over the world. But simply attending the conference is usually not enough....young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face...
  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...
  15. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...Eventually I learned that it’s practical because there simply isn’t enough time to read every single paper. I...present it all in one place to get a sense of the similarities and differences among proteins in the class....
  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...to use the ICE software tool is quick and easy. Simply upload your Sanger sequencing files and provide...analysis, which compares hundreds of samples simultaneously.  The current version of ICE software can analyze...
  17. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... of interacting and is much more effective than simply sending a message asking how things are going. ...
Showing: 301 - 320 of 591 results