We narrowed to 354 results for: Sele

1. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   Published

   July 1, 2022, 1:15 p.m.

   ...start can save a lot of trouble in the long run.  Selecting the method Once you’ve answered the above questions...

2. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   Published

   July 1, 2025, 1:15 p.m.

   ...Best Way to Elute and Store Your Plasmid DNA? Selecting Your Plasmid Purification Kit Resources on Addgene.org...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...Fusing FP fragments to your PIPs requires careful selection of FP fragments and, preferably, some information...

4. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ..., Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer...

5. Simplify Cloning with in vivo Assembly

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   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...specifically cuts methylated DNA, so it will selectively destroy the template, but not newly synthesized...

6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...linearly distant regions close together. gRNA Selection and Filtering for Off-target Effects We first ...

7. Plasmids 101: Colony PCR

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   Blog Post

   Published

   May 12, 2016, 2:30 p.m.

   ...make sure to use the appropriate antibiotic for selection.   Lysing bacteria and setting up PCR reactions...

8. CRISPR Antimicrobials

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   Blog Post

   Published

   May 3, 2016, 2:30 p.m.

   ...resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria, allowing them to multiply...

9. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ... histidine-auxotrophic host strains are used to select for binding-dependent expression of the HIS3 gene...

10. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ...above. There’s many variations in how one can gate, select, and analyze data. But now that you can read a ...

11. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...asked whether this step can be skipped or whether selecting colonies and performing Sanger sequencing will...

12. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...will fight more adamantly for their solution), selecting a devil’s advocate is a good option for any meeting...

13. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...region of your final plasmid or those you are selecting for further assemblies. This will help confirm...

14. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting the Right Antibody Antibodies 101: Polyclonal ...

15. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...ability to rapidly detect nucleic acids with high selectivity and sensitivity from patient samples. Find the...

16. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ...research for single or multiple disciplines – you can select the disciplines and the years. Which leads to the...

17. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ...GC’ sequence context. If this cannot be avoided, select the target AID version. Expect very low to nil ...

18. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ... PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging of tRNA isoacceptors induced by amino‐acid...

19. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...balanced with predicted off-target activity when selecting guides. Guides were delivered as a pooled library...

20. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...Addgene offers vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of...