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...protein phosphatase that responds to rising intracellular calcium levels within the cell. When levels ... wild type (top panels) and cells with high intracellular Ca2+ (bottom panels) equipped with destabilized...

...Therapeutics, which was acquired by Ultragenyx Pharmaceutical in 2017. As one of the original employees at...impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the...

...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or...

...possible. Instead of crowding the interface with every possible option, we place the most important controls...

...contacting YNP staff to arrange permits to visit places not accessible to the public and retrofitting our...protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between...

...Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable...

...toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy Patrick  Listen to...nanobodies to study retrograde transport from the cell surface. Their toolkit includes a set of plasmids each ...

...scientists around the globe. As a member of the Brassicaceae family, Arabidopsis is related to a variety ...pots, trays, soil, fertilizer, seeds and a growth space with fluorescent lights. Other experiments require...

...self-promoting—No one is going to tell you to pick up the pace Constant worry about grants and renewals What is... can be an issue in getting promoted (especially race and gender) Interpersonal issues can create interpersonal...

...Incubate 5 Minutes at Room Temperature: You can place your reaction on ice if you are planning to transform...polymerases with proofreading functionality are used in place of Taq to reduce error rates; however, proofreading...

...professorship. But recently a downward trend in funding and space in academic research labs has more students looking...industry, and government on social media (Twitter, Facebook, etc.) to learn about available jobs, fields, ...

...help researchers working on COVID-19. Its origins trace back to March 2020, when the first coronavirus lockdown...appealing, join us and help make science a better place. You will receive opportunities to collaborate with...

... our mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...reproducibility best practices in your research. For a place to start, check out Reproducibility for Everyone...

...Return of Race Science is a terrifying and equally good wake up call about the science of race."     - ...

...susceptibility of biofilms formed on various structured surfaces. Plasmids expressing fluorescent proteins will...biomarkers, and recently, cancer research related to space medicine. Now he aims on tracking pollution in rivers...

...movement. When the ribosome moves along an mRNA, it displaces other RNA-binding proteins to allow translation...translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal:...

... hires initially based on those who notice and embrace the “odd and extreme openness” of the lab (i.e ...are engrossed in a task. Things seem to fall into place frequently and you sometimes feel confident in your...

...amplified to add 5′ and 3′ homology regions to the adjacent fragment or vector. 5′ overhangs are generated...higher temperature at which Gibson assembly takes place may limit formation of secondary structures at the...

...can see from Figure 2 that determining where to place your gate based on the dot plot would be complicated...Conventional vs Spectral Flow Cytometry Beyond Surface Labeling in Flow Cytometry Resource on addgene....

...endeavor. Due to the COVID-19 pandemic, students will face a reduced supply of internships as a result of the...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...