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We narrowed to 816 results for: ache

Showing: 301 - 320 of 816 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...scientists actually loving the MTA?! Visibility and outreach Scientists know about Addgene and our visibility...increasing internationally, thanks to evolving outreach tactics, increasing business development efforts...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

  2. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...finishing your thesis as it is for helping your team reach an important milestone at work.   While I was in...always found that taking a conscious or active approach can be hugely helpful not only for developing ...can start by paying attention to how your mentors reach solutions and ask them to explain their logic. Or...about technical know-how and more about how you approach new challenges.     Know your strengths! Research...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...intended edit further weakened recognition by MMR machinery and improved editing efficiency. The lab also ...

  4. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of... If you want a plasmid for free, you can always reach out to the PI or corresponding author of the paper...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...

  5. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively...continual advancements in gRNA design necessary to achieve maximal on-target and minimal off-target activities...lentiviral plasmids containing one or more gRNAs. As each ligation reaction uses a pool of gRNAs as starting...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...target sites for base editing (Kim et al., 2017). For each base editor, they observed editing activity with...sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells, ...Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of a fusion...activity compared to ABEmax. In an alternative approach, engineering and directed evolution of the TadA...10.1038/s41587-022-01533-6 Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki...

  7. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...whether two proteins are within 10 nanometers of each other, or measure the tension in the helical structure...the fluorophores must be located within 1-10nm of each other and be oriented appropriately for energy transfer...Intermolecular FRET can be experimentally difficult to achieve, because the ratio of acceptor to donor fusions...PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...which uses a much shorter crRNA. Cas12-RNP-PAGE achieved high editing frequency (nearly 100% in some cases...method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with multiplexed...successful in primates. The authors used a multispecies approach to generate and screen a library of AAV9 variants...best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier and delivering a transgene...

  9. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...These therapies are delivered with an autologous approach, editing patient cells ex vivo and then returning...but especially concerning when using a systemic approach. Regarding effectiveness, if the editing frequency...meaningful way. Delivery, especially for systemic approaches, has its own unique set of obstacles. Thus far... liver. Similarly, for cardiovascular disease approaches targeting cholesterol, these particles were an...

  10. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...brain structure and function, with the advent of each tool expanding our ability to study and understand...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...

  11. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...way, but rather just labels the whole brain. To achieve labeling of only first-order connections to the...cell type specificity of the starter cell can be achieved by using EnvA-pseudotyped RABV, and only expressing...supplied in trans. Monosynaptic RABV spread can be achieved by modifying the wild-type RABV genome, which ...previously thought. The differences in the inputs to each of the two populations could be used to explain ...

  12. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...non-functional fragments. When these two fragments are near each other, they combine forces to form a functional ...complementation assay (PCA). In the Split-BioID method, each BirA* fragment is then fused to one of two bait ...and Z interact, the two BirA* fragments complement each other to form a functional BirA* enzyme. When this.... (2017). Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...

  13. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... newly synthesized RNA from the transcription machinery. Terminators are found downstream of the gene ...three RNA polymerases (Polymerases I, II, and III), each responsible for transcribing different types of ...termination are understood and it has been noted that each eukaryotic RNA polymerase terminates differently.... PubMed Central PMCID: PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer...

  14. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques ...promoters which will create unique mRNA transcripts for each gene that is expressed, multicistronic vectors simultaneously... that only a single translation event occurs for each mRNA. However, some bicistronic vectors take advantage... some of these peptides, no consensus has been reached on which peptide works best. Likely the choice ...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...the genome. Native Cas9 has two catalytic domains, each of which cleaves one strand of DNA upon binding ...proteins derived from different bacterial species, each of which interacts with a distinct gRNA that allows...expression with truncated gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying...engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826. PubMed ...

  16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... of the post! Speed: Your grant deadline is approaching or your advisor is demanding results. If you’...Cost: Funding is usually limited - what cloning approach should you use if the budget is particularly tight...however, or simply looking for a more streamlined approach, you might want to give Gateway a try. This method...answer, so don’t be afraid to explore multiple approaches to find out which one works best for you. Happy...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...vectors can be made at high titers equal to those achieved with lentivirus but offer an improved safety profile...increases the possibilities when designing screens. Each serotype has its own tropism, or a preference to... you can use smaller volumes of your library for each experiment. Additional considerations One of the...Performing In Vivo CRISPR Screens Using the FITS Approach Additional resources at addgene.org CRISPR pooled...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...to, I made it a personal goal to ask one question each day (this may be more or less difficult depending...identifies a potential collaborator from the meeting, reach out to them, pointing out that you heard their talk...handles to help you find other scientists attending. Reach out if you’d like to meet someone and set aside ...to set goals for myself like meet ten new people each day. Exchange business cards. When you’re done networking...

  19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ... the human genome with fluorescent proteins. For each gene, we needed to design guide RNAs, donor templates...gaussian normalized cut-to-insert distance) This gives each guide a score ranging between 0 and 1. The cutting...target. The CFD score ranges between 0 and 100 for each guide, with 100 being the strongest interaction ...but the protein sequence they encode is not. After each mutation, it will check the CFD score. If it is ...

  20. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...Genentech, will not. The point is that each company has a unique approach, and some might suit your needs better...setting—especially if job applications require teaching experience or high numbers of publications, which...into the general careers section of their web site. Each position is for a specific, pre-approved project...
Showing: 301 - 320 of 816 results