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We narrowed to 420 results for: genome

Showing: 301 - 320 of 420 results
  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...cells), or they can also be integrated into the genome by cotransfecting them with a plasmid expressing...Lett. 110, 073704 (2017). Hurt, R. C. et al. Genomically mined acoustic reporter genes for real-time in...
  2. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...which can become costly and impractical for whole-genome (and often, whole-plasmid) sequencing.  Prior to...well as the ability to sequence an entire viral genome in one read. We also use Nanopore sequencing to...
  3. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...alone, and this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now...22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial plasmids: classification and identification...
  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...  IPTG-inducible expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ:...expresses T7 RNA polymerase (RNAP) from the bacterial genome under control of the lac repressor, which is inducible...
  5. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. Ahmed A. Gomaa, et...
  6. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...flanking regions comprised of upstream and downstream genomic sequences (Figure 2B).   Figure 2: A comparison... boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. When it...
  7. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (...Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org Checkout...
  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ... Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using...
  9. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...technology, researchers are having trouble getting NgAgo genome editing up and running in their labs. Pooran surveyed... Genes: How to Experiment with Enhancers and Epigenomics 5 Tips for Troubleshooting Viral Transductions...
  10. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    .... Structural biology of CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20...
  11. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...cytosine base editor, BE3, induces a low frequency of genome-wide off-target DNA editing. Using several rapid...
Showing: 301 - 320 of 420 results