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We narrowed to 415 results for: genome

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Showing: 301 - 320 of 415 results

  1. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...  IPTG-inducible expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ:...expresses T7 RNA polymerase (RNAP) from the bacterial genome under control of the lac repressor, which is inducible...

  3. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. Ahmed A. Gomaa, et...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (...Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org Checkout...

  6. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...flanking regions comprised of upstream and downstream genomic sequences (Figure 2B).   Figure 2: A comparison... boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. When it...

  7. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ... Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using...

  9. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...technology, researchers are having trouble getting NgAgo genome editing up and running in their labs. Pooran surveyed... Genes: How to Experiment with Enhancers and Epigenomics 5 Tips for Troubleshooting Viral Transductions...

  12. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...cytosine base editor, BE3, induces a low frequency of genome-wide off-target DNA editing. Using several rapid...

  14. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    .... Structural biology of CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20...

  17. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...CRISPR’s effectiveness at high copy numbers and genomic loci with low accessibility. Furthermore, gene ...endogenous precursors of microRNAs found in the genome, whereas shRNAs are exogenously derived. Importantly...

  19. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Therapy Center, Associate Director of the Ocular Genomics Institute, and an Associate Member at the Broad...
Showing: 301 - 320 of 415 results