Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 678 results for: ids

Showing: 301 - 320 of 678 results

  1. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...efficiency. In the same vein, locked nucleic acids or bridged nucleic acids can also be introduced in the gRNA....consider include amide linkages, unlocked nucleic acids, and a constrained ethyl.  Can’t decide which RNA...While both have similar advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient...ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...24360272. PubMed Central PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui...25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui...27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira...26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional ...Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones...

  3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient cells during a tri-parental...RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the ability to synthesize diaminopimelic...your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M....

  4. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, ...epitope recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide...., who published a paper showing that the amino acids neighboring the Myc epitope influence 9E10’s affinity...Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...

  5. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ... human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside the ...bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., 2011). In...collection database, and it may also be aligned to plasmids in our inventory containing the suspected contaminating...Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright... AAV Find Ready-to-Use Lentivirus Browse Viral Plasmids ...

  6. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...More about Plasmids for Endogenous Gene Tagging in Human Cells Check Out the Michael Davidson Fluorescent... on the gene names in the figure below to find plasmids containing that particular gene. ...Prescott (Monash University). You can find more plasmids for labeling your subcellular structure of interest... for Cell Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent Protein Guide...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about...questions and alternative systems The ready-made PITCh plasmids available from Addgene are perfect for expressing...Protoc. 11(1): 118-33. PMID: 26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, ...” Cell Rep. 13: 621-633. PMID: 26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi...about CRISPR on Our CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid ...

  8. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...compared to transgenic animals (17).  These viral plasmids are available through Addgene.  The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References: H... Ross A, J. Hecksher-Sorensen, R. Baldock,  D. Davidson. (2002) Science, 296, 541-545. Pubmed. A. Ertürk.... Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et al. (2013) Nature, 497, 332-337. Pubmed. R....

  9. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ...the concentration and purity of nucleic acids, such as plasmids, the go-to method is to use spectrophotometry...wavelengths: 230 nm, 260 nm, and 280 nm. Nucleic acids exhibit a characteristic absorption spectra across...secondary peak around 280 nm thanks to aromatic amino acids like tryptophan and tyrosine (Figure 1B). The A260...assay or running an agarose gel. In the case of plasmids, the use of RNase treatment to degrade bacterial...

  10. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...can be encoded in the primer sequences. Multiple plasmids can be included in a single PCR, for example for... to the PCR mix. When PCR is not possible Some plasmids just won’t amplify! Examples of this are the GC-rich... GC-rich ITRs of AAV genomic DNA encoding plasmids, or promoters such as the chicken beta-actin promoter...cloning by homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID: 8346047...resources on the Addgene blog Read all of our Plasmids 101 blog posts Read our blog post on 5 factors...

  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry applications...modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple cloning...throughput cloning is to facilitate the construction of plasmids with many components. The creators of the MXS-...Assembly Learn About Other Plasmid Cloning Techniques Plasmids 101: Green Fluorescent Protein Resources on Addgene.org...nearest neighbor base sequences in deoxyribonucleic acids. The Journal of biological chemistry. 1962. June...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...transformation Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...localization of your protein of interest. Find all plasmids from the Lee-Parsons lab. Test different leaf...

  13. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...mammalian species to produce gas vesicles. New mARG plasmids Our new mARGs come as a set of two PiggyBac integratable...genes gvpNJKFGWV marked with Emerald GFP. These plasmids can be used directly in transient transfection...several generations to dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction...set of transient constitutively-expressed mARG plasmids driven by CMV promoters. Labs that are new to ...which is the transient cotransfection of the mARG plamsids into HEK293T cells using a chemical transfection...

  14. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...mediated Genome Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038/mtna.2016.48 Katti...identify and enrich base editing activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/...APOBEC–Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar...editing enrichment (TREE) in human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

  15. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... an allelic exchange experiment? Find the pTOX plasmids here! Continued development of new allelic exchange...and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID: 24234441...systems for unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID: 25800749...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn...recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our molecular biology...

  16. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...Find Synthetic Biology Plasmids Find Bacterial Expression Systems Find Plasmids for Protein Purification...associated with phosphorylation. However, these amino acids fail to recapitulate the true steric and charge-... during translation. Many ‘flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked...2014: mutations, PTMs and recalibrations." Nucleic acids research 43.D1 (2015): D512-D520. PubMed PMID: 25514926...

  17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

  18. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ... labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus, we are...trends for Zinc Finger, TALE, CRISPR, and SynBio plasmids over time. You can see these data in the slide...molecular biology technology? Have you deposited your plasmids yet?      ...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...thumb, we avoid target sites that code for amino acids near the N’ terminus of the protein, in order to...Likewise, we avoid target sites that code for amino acids close to the C’ terminus of the protein, to maximize...dCas9-mediated transcriptional repression. Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583...scarless human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to ...CyOFP1-NLuc-CyOFP1; Antares). Direct injection of plasmids for Antares or firefly luciferase into mouse livers...Additional Resources on Addgene.org Browse Luciferase Plasmids Browse the Fluorescent Protein Collection Find...
Showing: 301 - 320 of 678 results