We narrowed to 403 results for: mag
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TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....as possible. We must note that CRISPR is not a ‘magic bullet’: its use will always come with some uncertainty...
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Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....you of anything? Perhaps the Scientific Method? Imagine your problem is just a question you have about ... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....system for delivering gene editing tools into cells. Image adapted from Segel et al. 2021. Prime editing... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....chemical reaction, can be detected using specialized imaging instruments or X-ray film and lends itself well... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....of 2 96-well microplates (identical copies, see image above). We then select forward and reverse primers... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....sufficient quality (error bars, statistics, clarity in images etc)? Did they perform all of the appropriate controls... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....single-stranded DNA stretch that is accessible. Image from Gaudelli et al., 2020. Directed evolution... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....enzymatic reporters for cellular assays and animal imaging studies. The ability to simultaneously alter multiple... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....agar in it. The agar sits on a hot plate with a magnet so that the agar doesn’t solidify while it goes... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m.... Figure 1: The key components of Nanoblades. Image from Mangeot et al., 2019. MLV GAG-POL polyprotein... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....contrast interference (DIC) microscopy and computer imaging processing. The combination of these methods provides... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....induce non-homologous repair at the site of DNA damage. These nucleases are microinjected into a zygote... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI. Here are ... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m....Workflow for high complexity Golden Gate Assemblies. Image courtesy of New England Biolabs. DAD Tools... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....important features such as influential citations, images, and key phrases. It also takes into account how... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog PostPublishedJuly 14, 2016, 2:30 p.m....editing. Find Cpf1 Plasmids at Addgene Image attribution: Zetsche, Bernd, et al. "Cpf1 is a single... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m.... Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping. PubMed PMID: 18614669...