We narrowed to 486 results for: mme

1. Management for Scientists: What Makes a Good Manager Anyway?

   Type

   Blog Post

   Published

   Dec. 9, 2014, 2:22 p.m.

   ...knowledge-intensive workers”. I have added a few comments of my own to this list. Need feedback on their...

2. Choosing Your Perfect Empty Backbone

   Type

   Blog Post

   Published

   Aug. 19, 2014, 3:39 p.m.

   ...backbone. Or just ask –  leave a message in the comments below and an Addgenie will help you find what ...

3. The Stingy Scientist: How the Baby Gel Box Was Born

   Type

   Blog Post

   Published

   April 15, 2014, 3:11 p.m.

   ...ingenuity to solve this problem.  None of the commercial boxes were small enough to minimize the required...

4. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

   Type

   Blog Post

   Published

   April 12, 2018, 1:02 p.m.

   ... in ordering from ABRC for the first time, we recommend reaching out to us at [email protected] for help...

5. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   Published

   Jan. 25, 2018, 2:09 p.m.

   ...mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor Xa loves calcium, but metal-chelate...

6. Identifying Sequence Elements with SnapGene's Feature Database

   Type

   Blog Post

   Published

   Dec. 21, 2017, 2:06 p.m.

   ... annotated boundaries for control elements in commercial plasmids were inconsistent and sometimes clearly...

7. The Strength of Story Telling

   Type

   Blog Post

   Published

   Dec. 19, 2017, 2 p.m.

   ...relationships are all things almost all of us are programmed to appreciate and understand. We build narratives...

8. Plasmids 101: Origin of Replication

   Type

   Blog Post

   Published

   Nov. 11, 2020, 2:23 p.m.

   ...something clarified? If so please, post in the comments.  References Chatterjee A, Sun SB, Furman JL, ...

9. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

   Type

   Blog Post

   Published

   Oct. 20, 2020, 1:15 p.m.

   ...that you've been working on, let us know in the comments below! Additional resources on the Addgene blog...

10. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...Limitations with GFP in imaging  While GFP has been an immensely powerful tool to study proteins in vivo, it does...

11. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    Published

    Sept. 5, 2019, 1:06 p.m.

    ..., office of knowledge transfer, office of commercialization Additional resources on Addgene.org Addgene...

12. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ... and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation filter...

13. New and Upcoming Viral Vectors - Spring 2019

    Type

    Blog Post

    Published

    March 26, 2019, 1:40 p.m.

    ... (pAAV.hSyn-FLEX.iGABASnFR.F102G) is noticeably dimmer than iGABASnFR, and accumulates in the endoplasmic...

14. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type

    Blog Post

    Published

    Jan. 24, 2019, 2:24 p.m.

    ...large brain samples. For large brain samples, we recommend pretreatment with ScaleCUBIC1 (Susaki et al., ...

15. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type

    Blog Post

    Published

    Sept. 21, 2023, 1:15 p.m.

    ...model system is a mouse, given the wealth of commercially available mouse monoclonal antibodies. The good...

16. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. (2003) 60...

17. Antibodies 101: Chimeric Antibodies

    Type

    Blog Post

    Published

    April 25, 2023, 1:15 p.m.

    ...anti-Protein B antibody (Fig. 3)    Figure 3: Recommended secondary antibodies for rat IgG1 and mouse 1gG2a...

18. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ...using NgAgo? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions...

19. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...piece (see NEB for more information). Thus, we recommend that you add 3-6 bases upstream of your restriction...

20. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ...In the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for...