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We narrowed to 689 results for: mos

Showing: 301 - 320 of 689 results
  1. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...same chemical reaction performed by CBEs. However, most CBEs include an inhibitor of base excision repair... a C → G editing efficiency of up to 68% at the most favorable target sites. Differential cytosine editing...on to develop more efficient and precise editors. Most researchers agreed on the first part of the likely...
  2. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...the CTD (also known as the PI), while REC includes most of the bridge helix. Created with BioRender.com....conformation frequently (Osuka et al., 2018), but it’s most often shown in an “open” state, with the NUC and...region is disordered, Cas9 binds DNA very weakly, most likely through unstructured electrostatic interactions...
  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...alternative approach is RNA interference (RNAi). The most common method is transfection of short interfering...protein), which has been designated CD365 in the most recent HLDA workshop (HLDA10), as an example. For...cell lines using these two antibodies. This is the most usual level of evidence supplied to support the ...
  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...such as malaria and toxoplasmosis. Despite their importance to global health, most apicomplexan genes ...bidirectional promoter, integrate at 11 specific chromosomal locations, and stably express the integrated ...are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for restoring...
  5. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más emocionados! Después de recibir la traducción...científica profesional, L. Denise Roche, para editarla. Estamos orgullosos de anunciar que CRISPR 101: Una herramienta...
  6. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...instructions to navigate various lab processes.  Our most recent protocol video detailing Streaking Plates...you navigate Addgene’s vast online resources. Our most popular How-To video on “How to Order” details every...
  7. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...in Ghana and in other developing countries” and “Almost all cervical cancers are caused by persistent infection...get plasmids to the researchers who need them the most. Check back here to learn more about future awardees...
  8. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...tribulations of the modern PhD program. Don’t be fooled! Most senior graduate students are friendly and full of...University of California, Los Angeles. She enjoys chromosome biology and reading Ben Vincent's tweets.    ...
  9. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...readers. Below I’ve compiled a list of the Top 10 Most Viewed Posts on our blog from the past year. A [email protected] if you are interested. Addgene's Most Popular Posts of 2014 How to Design Your gRNA...
  10. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...CRISPR plasmids and 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably...from 231 countries. Guides and protocols were our most popular content, while our beloved blog received...
  11. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...dug into the data from our repository to find our most requested plasmid in 2015. In line with all of the...year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015 was...
  12. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...You are finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You ...of nonacademic science careers is that a team is almost always involved. Demonstrating your ability to ...
  13. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ... the kit in December, GreenGate includes the six most frequently used elements in plant expression cassettes...generate multi-construct transgenic plants. In the most practical terms, what GreenGate can do is “save ...
  14. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...feedback from the team. Successful management can almost be boiled down to one, key concept: Creating a ... style.   Seeking feedback frequently One of the most important paths of information flow is the one that...
  15. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...organism I study, to be able to see it all scales. Most modern biologists focus on one aspect alone. They...requires an awful amount of skill,  time for which most researchers wouldn't have. They are motivated to...
  16. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells using zinc finger nucleases...
  17. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...communicate exactly what a site offers to their users, and most importantly, allow those users the ability to quickly...upgrade? Blugene now has a full expression. You’ll mostly see a friendly smile, but occasionally you might...
Showing: 301 - 320 of 689 results