Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 745 results for: nes

Showing: 301 - 320 of 745 results

  1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...cloning uses short sticky ends to insert genes into plasmid backbones, base pairing between these ~4 base ... different types of PCR-generated inserts. By harnessing the power of DNA repair, you can assemble multiple...2024.  References and Resources Further reading Harnessing homologous recombination in vitro to generate...

  2. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...learn new tasks and beat humans at the ancient Chinese game Go). In addition, there was an entire story-telling...neuroscientists are using techniques from across different disciplines to better understand neurobiology and human cognition... provide a highly versatile means of delivering genes to many different cell types with minimal immunological...effects. AAVs are used in experiments targeting genes to specific cell types in the brain, mapping neuronal...

  3. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...and funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity...variable actually makes a big difference for the robustness of the findings.   How do we make research more...code, and other materials. The use of reporting guidelines can also help remind us to include all of the...

  4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...PAM sequence that the Cas nuclease recognizes determines site accessibility. Cas9s with a broader PAM ...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...

  5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...observe a phenotype, the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are commonly... al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms tRNA-gRNA and tRNA-gRNA-tRNA...constructed PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs...knockout, or multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also...

  6. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...clinicians, researchers, and even farmers are harnessing these molecules for good. RNA:RNA dsRNA and ...exploited for antiviral purposes similar to that of vaccines. If you know the sequence of a virus, you could...dsRNA. Synthetic dsRNA Researchers have been harnessing the power of dsRNA for a long time, even before...complete. However, transcription of thousands of genes is constantly occurring throughout a single cell...

  7. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et al., 2017). However, Acr inhibition...What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires...compatible approach to simultaneously clone two genes-of-interest (GOI) into two independent expression...

  8. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion-pump encoding genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction...fluorescent protein. This GEVI features increased brightness and improved plasma membrane specificity. Stable...encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID...

  9. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...definitely not worried. I have confidence that from Business Development to Shipping we will execute any new...thanks to evolving outreach tactics, increasing business development efforts, and our London team. We show...Atlantic. We’ve been in documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. ...dedication, flexibility, innovative thinking, and openness to team work.   Company culture Once in awhile...

  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Chen et al. successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the...of fluorescent puncta observed. By labeling two genes of a given chromosome simultaneously, they could...also monitor the spatial relationship of the two genes over time, at a range of distances from 2-75 Mbp...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...Science Foundation, and Stanford University—that harnesses the creative power of sex and gender analysis ...sex of their cells. For one thing, cells and cell lines degenerate over time—and sex can be hard to determine...indicating the sex of the most commonly used cell lines appearing in American Journal of Physiology-Cell...Engineering, and Environment—a project aimed at harnessing the creative power of sex and gender analysis...

  12. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...directions of the project, Outlines a detailed scope of specific aims, milestones, possible setbacks, and...will address these limitations and lead to new milestones in the field. Research strategy The Research...proposals consist of 3 to 5 aims, with secondary milestones to meet along the way to achieving each aim. ...

  13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...in your career path and still find comfort and happiness there. Read all of the Meet an Addgenies features...Fabienne Furt, Addgene’s Associate Director of Business Development, got her PhD in Plant Biochemistry...she joined an investment club and discovered the business side of science, she stuck with it. Lab Manager...people, and making them get outside of their comfort zones. I came to Addgene with a goal to learn nonprofit...

  14. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...that takes advantage of DNA sequencing to turn mAb clones into expression constructs to be conveniently modified...long-term solution to reproducibility and cost-effectiveness for broad and large quantity uses. We therefore...accuracy while screening. We also compare different clones of the same target to see if the results are the...primary antibody compared to negative control also determines the signal’s strength. Exposure time is the amount...

  15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...spike protein can be used for the development of vaccines and diagnostics (Hsieh et al., 2020). As the virus...diagnostic enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase and Taq..., from Addgene to better understand which human genes are important for SARS-CoV-2 entry into human lung... information on these plasmids and a behind-the-scenes glimpse of their work, head over to their blog ...

  16. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...cells, one may have the unfortunate experience of witnessing…nothing. For some unclear reason, cells are not...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic...conditions for storage, transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603...receptor-independent virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. ...

  17. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... such as LTRs, gag, WPRE, antibiotic resistance-genes, or the transgene itself. Many researchers prefer...that target the common features of viral vector backbones; once validated these universal primers can be...popular approach utilizes the antibiotic resistance genes carried on transfer plasmids. Target cells are transduced... for predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed PMID: 15291957...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...molecular biology toolbox to identify new target genes for drug discovery or to understand the intricacies...the targeting of hundreds to tens of thousands of genes with minimal effort. The versatility of Cas effectors...constraining for some experiments, particularly in vivo ones. In those cases, AAV vector screens may be AAVantageous...). Next-generation sequencing (NGS) to identify genes of interest. Figure 1: Overview of in vitro...

  19. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...other, you should be able to draw perpendicular lines that intersect your populations. If you see a diagonal...needs to be shifted, consider your perpendicular lines. In this case, the population on the left is a horizontal... Inputting compensation values differs between machines or the program you’re using, but usually you can... the plot. However, because we can see straight lines in the populations in the other corners of the dot...

  20. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...extra clips to hold up your poster, and pack a few business cards to tack up next to your poster. Arrive early... won’t know every answer – don’t hesitate to be honest and tell your audience just that. Explain what ...around the posters and read the titles to find the ones you’d like to revisit. Strategically traverse the...with the presenter after the session. Exchange business cards or jot down their name and affiliation from...
Showing: 301 - 320 of 745 results