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  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...individual colonies growing out in the 96-well dish. My protocol with many additional tips can be found here. ...knockouts and knockins in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...Organic dyes are typically both brighter and more photostable than fluorescent proteins (often up to 10-fold...

  3. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...possible in plants outside of limited, single cell protoplast analysis. Instead, microscopic plants must be...organisms with functional APT will convert 2-FA to cytotoxic nucleotides, resulting in death. In the presence...

  6. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

  7. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever...10.1016/j.cell.2014.02.001 Osuka, S., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima, T., Nureki...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...in rats. For more information on reagents and protocols, visit https://clover.caltech.edu. Figure adapted...and targeted gene delivery in rodents. Nature Protocols;14, 379–414 (2019). https://doi.org/10.1038/s41596...

  11. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...YouTube channel for more helpful animations, useful protocol videos, and quick tips for lab success.  ...

  12. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ... blog, including our antibody guide, antibody protocols, and Antibodies 101 blog series.  Stay tuned for...

  13. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...subscribe to our YouTube channel for quick tips, protocols, animations, and more, or follow us on TikTok ...

  16. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ... creative scientists delivered some spectacular photos once again this year! All winners receive an Addgene...

  19. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...Addgene.org Browse our Video Library Check out Our Protocol Pages Find Molecular Biology Resources ...
Showing: 301 - 320 of 495 results