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We narrowed to 509 results for: otos

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Showing: 301 - 320 of 509 results

  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...activity. Researchers have designed a variety of photoswitchable elements into many other types of proteins.... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...

  3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...Lentiviral Guide and our lentiviral production protocol.  One thing to keep in mind is that titering these... these IDLVs may require a slightly different protocol than regular lentiviral vectors. This is because...

  5. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...the application of this approach is limited to protoplast cells (plant cells whose cell wall has been removed...recommend reading the associated publications or protocols to get the most out of the plasmid you chose for...

  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...Find other tips and tricks for molecular biology protocols Browse other articles related to antibodies Resources...Resources on Addgene.org Find written and video protocols Visit Addgene's antibodies collection ...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... Require Ex Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002... CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment...Activated By Designer Drugs) to determine the role of serotonin in movement. Follow her on twitter @katrinaboards...

  9. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...individual colonies growing out in the 96-well dish. My protocol with many additional tips can be found here. ...knockouts and knockins in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene...

  10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...possible in plants outside of limited, single cell protoplast analysis. Instead, microscopic plants must be...organisms with functional APT will convert 2-FA to cytotoxic nucleotides, resulting in death. In the presence...

  11. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...protein to a DNA-editing enzyme to generate the prototypical base editor enzymes. The cytidine base editors...engineering using the CRISPR-Cas9 system. Nature Protocols 8:2281–2308 . https://doi.org/10.1038/nprot.2013.143...

  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...Organic dyes are typically both brighter and more photostable than fluorescent proteins (often up to 10-fold...

  15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever...10.1016/j.cell.2014.02.001 Osuka, S., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima, T., Nureki...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...in rats. For more information on reagents and protocols, visit https://clover.caltech.edu. Figure adapted...and targeted gene delivery in rodents. Nature Protocols;14, 379–414 (2019). https://doi.org/10.1038/s41596...

  20. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...YouTube channel for more helpful animations, useful protocol videos, and quick tips for lab success.  ...
Showing: 301 - 320 of 509 results