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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 301 - 320 of 628 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...only a small number of glutamate molecules are released in each synaptic event and they are rapidly taken...Institute for Protein Innovation (IPI) recently released a set of highly-specific recombinant antibodies...efficiency and lower indel formation than methods relying on homology-directed repair (HDR), and insertion...

  2. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...stay around? Is it too long or do they leave prematurely? Do lab alumni stay in the same field taking...Does the PI keep up on the literature and pass on relevant papers? Will you have access to information from...

  3. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...Many intracellular sensors of events like calcium release have been made using fluorescent resonance energy...biosensors, the intensity of the FRET signal can be correlated to specific concentrations of biological molecules...et al. (7) wished to trigger intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...ligase, conditions and suitable probes, LDR can reliably detect single-base differences. The original creators...interrogation of multiple potential SNP sites (15). The related LCR technique uses two sets of probes to achieve...DNA library preparation. However, when a method relies on accurate ligation of nicks lacking gaps or mismatched...

  5. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic liposome) or electrical...Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant and...viral capsid antigen p24, the FACS dosing method correlates viral titre to the number of cells transduced...

  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Biochemist, we geneticists are a lazy bunch who love to rely on the awesome power of genetic selection. Knock-in...recovery of these edits. Initial work in C. elegans relied on selective markers such as drug resistance (Chen...geneticist, I developed a co-selection approach relying on repair of a conditional-lethal mutation to identify...

  7. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... give advice and guidance to those that ask. Relationship building is key in science policy. Luckily, ...happy hours that you can attend to help build relationships.  Reach out to a science policy professional..., communications, diplomacy and international relations, advocacy, and writing legislation (see Figure...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...for dinosaurs wreaking havoc in a theme park.  Released in 1997, GATTACA is an American SciFi film starring... breaks (DSBs) in the genome. These technologies rely on the cellular DNA repair machinery, which can ...the adenine base editor (ABE) enzyme in 2017. ABEs rely on a similar strategy as CBE but use a ssDNA adenosine...

  10. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...inhibited by the unfolding of LOV, allowing the release of Cas9. A noteworthy application of the CASANOVA...in U2OS cells. Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later...Niopek D (2020) Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology...

  11. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process...page is to make it easier for you to find content relevant to your research and career goals, or to answer...

  12. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...Cervical Cancer is the leading cause of cancer related morbidity and death among females in Ghana and ...for cervical cancer development and also better correlate the presence of certain HPV subtypes with risk...

  13. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ... is in a top-tier publication. Accessibility is surely also behind the increase in citations for older...scientific discourse has increasingly moved online, surely times have changed. Altmetrics.org calls to expand...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... may need to sonicate your lysates in order to release the proteins from their binding. If you’re seeing...technical replicate (ie, leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget...migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins of vastly...

  15. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...protein domains from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and ...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells."...

  17. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...beneficial effects on the brain,” Ruas said in a press release. “We actually found the opposite: well-trained ...doi: 0.1016/j.cmet.2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

  18. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A ...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...

  19. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... I actually started at a later date and worked entirely virtually. Stepping into this new virtual world... departments of the company. I asked questions relating to their roles at Addgene. I had a great time ...

  20. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...up hobbies (for her its gardening) that help you relax and enjoy life. “I love academics because it offers...other's accomplishments? Any tips on making time to relax and/or enjoy time away from the bench? We would ...
Showing: 301 - 320 of 628 results