We narrowed to 855 results for: NTS

...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x... and electronics systems, FACS instruments have specialized components that can divert a subset of cells...overcome these challenges and make these types of experiments easier and faster to perform. Introduction to...channel number based on intensity. Higher intensity events are assigned to higher channels. In a typical assay...assay a user will compare the shifting intensity of events. Since intensity positively correlates with expression... flow cytometry particularly well to complex experiments looking at a variety of different targets. If...for setting up antibody based flow cytometry experiments.       References and resources References:...

...analyzing thousands of edits performed over multiple experiments and comparing the robustness, accuracy, and speed...also provides a Knockout score (KO-score) which represents the proportion of cells that have either a frameshift... How ICE works   Following delivery of CRISPR components into target cells, genomic DNA from both edited...analysis, which can compare up to five editing experiments at a time, and batch analysis, which compares...confident you can be in the ICE score. KO Score - Represents the proportion of cells that have either a frameshift...the edited pool. The black vertical dotted line represents the cut site, and yellow + symbol on the far ...trace. The horizontal black underlined region represents the guide sequence, along with the red underlined...

..., case studies, and experiments for labs, in addition to testing those experiments. “We also do videos... with students and faculty, Caitlin also collaborates with the sales and operations departments at eScience... on her job as an SME: “I write curriculum for students taking science courses online, which is a blast...then condensing that and making it accessible to students.” Making science approachable is often the most...in isolation. “A lot of what we do is work with students who don’t have a science background. [We ask them... demonstrating an experiment or a technique to students, like how to pour a gel [for] electrophoresis ...team to meet faculty and introducing the kits to clients who might initially be doubtful of online science...

... are key elements of an AAV plasmid. Parts shown in grey are optional cis-regulatory elements.   Key...sequences. Other cis-regulatory elements Next let’s talk about some of the elements that aren’t required parts... common AAV plasmid elements! And while there are many other AAV plasmid elements and combinations out... Where do you start? Turns out like all good experiments, making AAV starts with some plasmids. You just...Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...Addgene blog Learn more about basic viral vector elements Read about four considerations for the perfect...

...OCRL1 gene that leads to the early death of young patients as their kidneys fail. The condition was first...piqued their interest. They obtained cells from patients with the condition to reveal some “outstanding...problem. “We’ve had the opportunity to meet a few patients that suffered this disease,” Aguilar said. “Once... (XP). This recessive genetic condition leaves patients incredibly sensitive to sunlight and prone to ...interested in XP out of a desire to understand the early events in the transformation of healthy cells into melanoma... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire... as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC suffer from recurrent attacks ...

...homologous DNA fragments in a single-tube reaction. For mutational scanning experiments, libraries of ...of a plasmid library that expresses all desired variants of a protein. Applying a selective pressure winnows...winnows the pool down to plasmids expressing variants with optimal function. High-throughput DNA sequencing...mutational scanning Hundreds of thousands of protein variants can be tested in one experiment. There are many...of DNA fragments that contain variable bases (NNN) at any given codon site of interest can be cloned into...deficient in dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes...across a region of interest, generating multiple mutants in one PCR reaction. Below is a step-by-step overview...

...cell lines. As I started planning out my own experiments in graduate school, I had to divide my time between...the long and highly useful tasks. The quick accomplishments will likely energize you. For example, let'...experiment fails, you’ll still have made small accomplishments each day that should help keep you content ...section below, it’s important to dedicate specific amounts of time to specific tasks beforehand and assess...my undergrad days, I had general goals for my experiments, but didn’t spend enough time thinking about ...quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time for different...make sure I have explicit goals in mind for the endpoints of a task or project (with metrics if possible...

...condition 1” points (POI and loading control) are smaller than the analogous green “condition 2” points by the...blot.   Total protein measurements as loading controls Total protein measurements are an accurate alternative... example gel (left) with bands corresponding to points on the plot of protein abundance versus band intensity...the exact same value. For the two solid POI points, this difference is reflected on the band intensity...intensity axis. But the loading control points are outside of the linear detection range. As a result, they have...Outside the linear detection range, band intensity measurements become extremely susceptible to error, since...for your POI. Figure 6: Total protein measurements are taken on the same gel or membrane (depending...

...all the variant PUF segments required to bind any 8 base RNA sequence. The segments are provided in Gateway... have any questions, please write to us in the comments section.  FX cloning kit This kit is designed...Translation of the standardized leader peptide prevents secondary structures in the mRNA encoding your...expression systems; the Cell-free expression test kit represents a set of 88 clones in a pCellFree vector that...quantitatively determine allele frequencies across timepoints or populations. This kit consists of a 48 plasmid...ordered progression to resistance." Antimicrobial agents and chemotherapy 57.11 (2013): 5373-5383. Walkiewicz...Assembly Kit PUF proteins are composed of repeat segments that can be engineered to bind specific RNA bases...

...fluorescent proteins, with recombination events specified by three loxP variants, but the principles remain the ...recombination to generate additional recombination events, and the synthesis of alternative loxP sites to...expression; these deletions are defined using 2 loxP variants (only identical loxP sites can mediate recombination...either deletion removes one of the other loxP site variants, preventing further recombination. Brainbow-1.1...) The construct contains two tandem invertible segments, each with two fluorescent protein coding sequences...combinatorial effect of multiple, independent recombination events leads to a rainbow of colors. With three copies...Variations Between Brainbow Systems   Brainbow variants and applications beyond the mouse brain In addition...