...non-functional, you may simply introduce a frame-shift mutation. This can be achieved using a restriction...standard ligation reaction resulting in a frame-shifted coding region. It is worth noting that unlike these...specifically designed mutations (not limited to frame shift mutations). Consequently, site directed mutagenesis...

...and respect your IP rights and data ownership? IP and data ownership should be yours (your institution’...sure you don’t use ELN providers that take any ownership over your data or have direct access to it. Your...

...GFP to be translated), and second, through a gel shift assay using the PhosTag system (see below). This...phosphate moieties and promotes a robust mobility shift in phosphorylated, but not non-phosphorylated proteins...done using mass spectrometry to identify a mass shift proportional to a single phosphate moiety on the...

...Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y., & Konishi, H. (2014). A comparative analysis...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-... . https://doi.org/10.1534/genetics.115.178335 Dokshin GA, Ghanta KS, Piscopo KM, Mello CC (2018) Robust...

...recombination genes at low temperatures (30-34˚C).  Shifting bacteria to 42˚C for 15 minutes quickly inactivates... is not required for maintenance. A temperature shift to 42˚C not only allows for activation of the red...Department of Laboratory Medicine at the University of Washington. She is particularly interested in science communication...

...remotely for the last year to give our lab and shipping teams enough space at HQ and for some of us, that...email from us asking for your mailing address and T shirt size. You have until April 7, 2021 to make your...

...PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and ...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa...

...countries. Just eight days into 2024, we've already shipped 2,078 items to 764 scientists at 375 organizations...different countries. It's safe to say we keep our shipping team busy! But they're not alone: to better serve...

... cannot be washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can ... striving to set an example of environmental stewardship.  Additional resources on the Addgene blog ...