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We narrowed to 466 results for: SHI

Showing: 161 - 170 of 466 results
  1. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...light of the policy, hold onto data longer before publishing. But there are strong incentives to publish such...data initiatives are also taking root. Nature Publishing Group recently launched a publication called ...veracity of collections and interpretation of data, publishing complete data, rather than just a single projection...
  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...multiple sources of error (red) influence these relationships and must be addressed with separate controls...accurately quantified In western blotting, the relationship between protein abundance and band intensity... BioRender.com. To see why this S-shaped relationship poses problems for proteins of very different...
  3. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...and respect your IP rights and data ownership? IP and data ownership should be yours (your institution’...sure you don’t use ELN providers that take any ownership over your data or have direct access to it. Your...
  4. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...differences, they had a hard time maintaining their friendships. I put “neutral” in quotations here because I...because that can indicate confusion around... Ownership: Misunderstandings about who is responsible for...for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork  ...
  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up... videos like this would prove to be difficult. Shipping the gear, and walking everyone through the recording...recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have multiple interview subjects...
  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...non-functional, you may simply introduce a frame-shift mutation. This can be achieved using a restriction...standard ligation reaction resulting in a frame-shifted coding region. It is worth noting that unlike these...specifically designed mutations (not limited to frame shift mutations). Consequently, site directed mutagenesis...
  7. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...GFP to be translated), and second, through a gel shift assay using the PhosTag system (see below). This...phosphate moieties and promotes a robust mobility shift in phosphorylated, but not non-phosphorylated proteins...done using mass spectrometry to identify a mass shift proportional to a single phosphate moiety on the...
  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y., & Konishi, H. (2014). A comparative analysis...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...
  9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-... . https://doi.org/10.1534/genetics.115.178335 Dokshin GA, Ghanta KS, Piscopo KM, Mello CC (2018) Robust...
  10. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...recombination genes at low temperatures (30-34˚C).  Shifting bacteria to 42˚C for 15 minutes quickly inactivates... is not required for maintenance. A temperature shift to 42˚C not only allows for activation of the red...Department of Laboratory Medicine at the University of Washington. She is particularly interested in science communication...
Showing: 161 - 170 of 466 results