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Showing: 321 - 340 of 450 results

  1. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., B.E., Gradinaru V. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology...

  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...covered systemic capsids, which allow AAVs to broadly spread throughout an organism, including difficult-to-...., Robbins, P. D., Li, J., & Xiao, X. (2006). Widespread and Stable Pancreatic Gene Transfer by Adeno-...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage for Antimicrobial Purposes...Systems to Probe Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org...

  4. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...RNAi to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate...the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large...

  5. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...each serotype also exhibits distinct patterns of spread from the injection site it is advisable to try ...off-target expression making it easier to determine the spread pattern around the injection site. Test the promoter...

  6. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...without telling you anything about outliers or data spread within those data sets. Measures of dispersion...allowance in a statistical test. Distribution - spread of data; typically, normal (bell curve) or skewed...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...by guest blogger Derek Simon. Optogenetics is spreading through the neuroscience community like wildfire...identification of opsin expression, extent of viral spread, and with co-localization immunofluorescence, cell-type...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...implanting them into female mice. Generating chimeric offspring shows the ability of the iPSC to contribute to... theses chimaeras to produce all-iPSC derived offspring demonstrates the ability of the iPSCs to form ...

  9. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...analyzing qPCR data, most with plug-and-play Excel spreadsheets available for easy analysis. These useful tools... vol. 1160, DOI 10.1007/978-1-4939-0733-5_3, © Springer Science+Business Media New York 2014Bustin S; ...

  10. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...eBook webpage.  More resources on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition...

  12. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you and your ...

  13. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...seminal” studies for various categories, including CRISPR gene-editing, the foundations of synthetic biology...

  17. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...showcases the five main topics of the blog: Plasmids CRISPR Viral Vectors Fluorescent Proteins Science Careers...

  18. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...submission includes: Images that clearly show viral spread and cellular tropism* (min. 300 dpi, PNG format...
Showing: 321 - 340 of 450 results