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We narrowed to 791 results for: Comp

Showing: 481 - 510 of 791 results

  1. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...service free of charge to scientists by allowing companies to pay publish their catalogs on the site. Like...of checking off each step as you painstakingly complete your experiment – one small victory in a long ...manage scientific data: Electronic lab notebooks compared with Google Docs and wikis - by Rory Macneil on...

  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...also is also called Bimolecular Fluorescence Complementation (BiFC). 8 points to keep in mind when choosing...divided by 1000. In many cases the brightness is compared to that of EGFP which is set as 1. Some proteins... BD bioscience. ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101...

  3. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...No examination of the state of science would be complete without the inclusion of some interesting case...Prashers will find it increasingly difficult to compete (for funding, etc.) with that produced by the confident...the U.S. environment, and a decline in the competitiveness of U.S. science and technology.” This last ...

  4. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...engineered DNA-binding molecule such as the CRISPR complex is expressed in cells to be analyzed, the Fujii...another "in vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and ... a genomic locus through a specific gRNA-dCas9 complex. When combined with next-generation sequencing,...

  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...your samples, the relationships between cellular components, and the accessibility of your target epitope...work by dehydrating and precipitating cellular components. This method of fixation is good for epitopes...away your target (for example, some cytoskeletal components) and you want to fix and permeabilize in one ...

  6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...cells can decrease the number of fluorophores, compromising the photon budget. Likewise, the switch from...labeling chemistry easier and more functional in complex biological environments such as live cells and ...brightness of the resulting conjugate, and the complexity of the system. A particularly desirable property...

  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very...may not apply. Table 1: The specific mutations comprising common fluorophores Fluorescent Protein Mutations...chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts the wavelength...

  8. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...decreased p-values of known ricin regulation genes compared to results generated using non-targeting guides...full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch...off-target sites, short guides had reduced toxicity compared to full-length guides. Reduced toxicity of short...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...the anticodon,  a three nucleotide sequence complementary to the mRNA sequence. The 3’ end of  the tRNA...resources (Athey et al., 2017) and many DNA synthesis companies, such as IDT, Genewiz and GenScript, offer tools...the other while other codons are under expressed compared to others. Image from Hiss et al., 2017. Most...

  10. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...1997). We have a lot of plasmids that contain components of the SB transposon system including those from...but think again. Spaghetti squash is actually a component of the fly actomyosin cytoskeleton that regulates...regulates cellular contraction. This network is composed of actin and myosin which interact with each other...

  11. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...C) installation by base editors. Pathways that compete with the desired outcome are not pictured. ...Plant base editor platforms and their molecular components. Adenine base editors for plant We developed...editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned above are...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...are differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to...sugars end up in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The... as described above and is inactivated by the complement system in serum (DePolo et al., 2000). Other ...

  13. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...laser cleavage and an RNA aptamer that bound the compound malachite green (MG) to create a non-protein based... 4-hydroxybenzylidene imidazolinone (HBI)-like compound allowing it to fluoresce, the Jaffrey lab asked...production have also made aptamers an important component of high throughput assays with microarrays and...

  14. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...both Addgene’s founding and our growth into the company we are today. As part of our 20th anniversary celebration...pages allowed researchers to easily access and compare their options for tools such as empty backbones...Addgene YouTube channel to add a strong visual component to our educational resources. From 2013–2023, ...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ... amplify well and others don’t. The size and composition of your library can drastically impact what conditions...suffice…and the answer is no. Due to the size and complexity of pooled libraries, NGS is the best way to validate... plasmids didn’t amplify after your screen is completed!  Producing Your Lentiviral Library Once you've...

  16. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...ordering the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...order them yourself through whatever DNA supply company you currently use. You can also find many previously...explored and it allowed control over each individual component of the system. However, it is easier to directly...

  17. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...line identity and purity making it easier for comparable studies to be performed on a cell line even decades...passage number, cell line, and date, which you complete each time you passage the cells (as well as recording...through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune, and Infors...

  18. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...needs a standard against which the results can be compared; results from an experiment lacking the proper...baseline/standard against which other samples can be compared. The Empty Vector Control (mentioned above) could... where expression of that gene is knocked out completely. These controls may or may not be plasmid-based...

  19. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...promoter present in your plasmid backbone must to be compatible with the type of RNA that needs to be made: if...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells... repression in the presence of glucose or by competitive binding of the anti-inducer fucose Weaker. Commonly...

  20. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...then following transduction, the partial genomes complement each other to restore the full-expression cassette... has equal or higher transgene expression when compared to using a single over-sized vector, but not always... one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such as a 77bp long...

  21. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...recombination intermediates can then resolve to complete the DNA repair process Homology directed repair...is elongated and ligated to fill the gap, thus completing SDSA.   Break-induced repair (BIR) pathway BIR... uses reverse transcriptase to generate the complementary ssDNA. Easi-CRISPR was shown to increasing editing...

  22. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...get people to remember you). What better way to accomplish all of these things naturally than to give a ...simplicity of their slides and how the slides complement the spoken message. Learn from their level of... topics that are unfamiliar to you because the company you are visiting works on them. Of course, if some...

  23. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...desirable. Should stem cell editing occur, the CRISPR components would only need to be expressed for a short period... with vastly reduced inflammation and necrosis compared to unedited animals. In grip strength, specific...

  24. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...system, Sanes and Lichtman also developed a complementary Flpbow construct that is functionally similar...technique will provide important insights into the complicated physical organization of the brain and other ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

  25. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... accessibility due to chromatin status). No computational prediction is ever perfect, but this can decrease...to conduct genetic screens, however, a library composed of the latter would likely be more effective, ...

  26. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nature Methods,...

  27. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...knowledge and skills had improved significantly when compared to their pre-program self-assesment. Achievements...other countries. In 2016, we expect the program to comprise over 2000 participants (mentors and mentees). ...

  28. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...transfer expert who understands the somewhat complicated Material Transfer Agreement process that kits...function. Project team A Project Team is assembled to complete a series of tasks with precisely defined goals...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...

  29. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus de l’Académie des Sciences - Series III ...

  30. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...expression, and is flanked (“floxed”) by two sets of incompatible recognition sites (e.g., loxP and lox511) in... Han, 2016). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could... "Two possible roles of bacteriorhodopsin; a comparative study of strains of Halobacterium halobium differing...
Showing: 481 - 510 of 791 results