We narrowed to 513 results for: EMB

1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron...

2. Healthcare Consulting: A Door to the Business of Life Sciences

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   Blog Post

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   Oct. 13, 2016, 2:30 p.m.

   ... on the expertise of the senior leadership and members of their consulting staff. Who would enjoy healthcare...

3. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...., Rubinstein, M. “LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis...

4. Plasmids 101: Control Plasmids

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   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central...

5. Tips for Using FRET in Your Experiments

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   Blog Post

   Published

   Nov. 5, 2014, 3:41 p.m.

   ...Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf...

6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   Blog Post

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   May 13, 2025, 1:15 p.m.

   ...luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus with secrets...

7. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   Published

   April 4, 2024, 1:15 p.m.

   ...running your analysis. In this case, I and other lab members had worked with lymphocytes before, so I knew where...

8. Transferable Skills Guide: Time Management

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   Blog Post

   Published

   Dec. 5, 2017, 1:52 p.m.

   ...physically cross things out (it just feels good) and I remember things better when I write them down. Some people...

9. Harnessing Bacterial Toxins for Allelic Exchange

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   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ...excellent efficiency using a commercial isothermal assembly master mix. Introduction of the strong transcriptional...

10. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    Published

    Oct. 11, 2016, 2:30 p.m.

    ...deliver these components to your target cells. Remember, efficient delivery of CRISPR components is essential...

11. Evolution of Lab Techniques

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    Blog Post

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    June 21, 2016, 2:30 p.m.

    ...smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization of a radioactively...

12. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi:10.1038/nature.2015.18947...

13. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Attribution License. Within the nervous system, remember that some serotypes can be transported retrogradely...

14. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...this might be frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed...

15. Plasmids 101: Dimers and Multimers

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    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ...control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which cannot distinguish...

16. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ...recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts used...

17. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...Director, Dr. Zafira Castano Corsino, a junior faculty member at Harvard Medical School/Brigham and Women’s Hospital...

18. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning...

19. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...handling cell lines. Avoid discussion with other lab members, sneezing or coughing in the lab. The human mouth...

20. Finding Your Science Policy Path

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    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...backgrounds. In fact, many of my AAAS STPF cohort members came to DC to do either a sabbatical year or transition...

21. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...liposome) or electrical (electric current forming membrane pores) transfection. These techniques are still...

22. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted in efficiencies...

23. Typing CRISPR Systems

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    Blog Post

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    March 18, 2025, 1:15 p.m.

    ...fact have a helicase domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation...

24. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...expression, CD45 is incorporated into the plasma membrane and can be detected through a cell surface label...

25. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ... cell mobility are essential processes during embryonic development. These are required for a growing ...

26. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...screen. Currently, eleven published libraries are embedded. These were kindly donated by six different research...

27. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...at a similar spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved...

28. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    Published

    March 25, 2025, 1:15 p.m.

    ...Base Editing. Advanced Science (Weinheim, Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002...

29. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...possible. Note that the full plasmid sequence can be assembled or theoretical — it does not have to be entirely...

30. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...October 2019 and updated by Emily P. Bentley in December 2024. References and Resources References Anzalone...