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  2. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...CRISPOR, a tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 ...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi...

  3. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...primary antibodies can be expensive and may not provide enough signal to visualize low-abundance targets...your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your primary ...

  4. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition...

  5. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...make them decide to read (or listen or watch) further.  Second, the introduction needs to provide enough ...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different...

  8. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...cells to take up protein directly? It’s not a new idea, but it comes with a few caveats. The most common...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...

  9. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

  10. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...control AAV inventory to give scientists access to a wide array of promoters and fluorophores for validating...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  11. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... schedule tools How to write a lot: A practical guide by Paula J. Silvia  This book has tips for how to...suggestions. De-jargonizer The De-jargonizer can identify jargon in your writing. While it’s not possible...

  12. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...inhibits over 18,000 human genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental... (Tet) On Expression Systems at Addgene Cre-lox Guide and Plasmids     ...

  16. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et... protein. This technique is very similar to the widely used FRET, but it eliminates the need for an excitation...

  17. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...synthesized endogenous proteins in situ. Now FUNCAT-PLA provides both quantitative and spatio-temporal information...protein synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...messengers. Optical control of kinase activity can provide greater spatiotemporal resolution than pharmacological...vanishing, and pa= photoactivation) domains on either side of the active site of a constitutively active MEK1...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...the respective hairpin binding domain. Pairs of identical hairpin sequences specify the primary colors blue... colors and a white composite. gRNAs with two identical hairpin binding domains produce blue, green, or...

  21. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ... excited about. Following scientists in fields outside your main research focus lets you keep a pulse ...window of time for publication. To help you quickly identify papers with greater influence, the average number...

  22. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... Kramer L, Campbell RE. Structure-and mechanism-guided design of single fluorescent protein-based biosensors...1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral...

  23. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein ... if that’s the option you choose. Once you’ve identified the linear portion of your standard curve, you...

  24. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...leadership and management skills. The NPA brought outside experts and many hands-on- and role-playing exercises...with other leaders in my research field should I decide to remain here.  In a time when it is so important...

  25. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...stranded break (DSB) at a genomic site specified by a guide RNA (gRNA) sequence. Users can design gRNAs to induce...SapTrap, scientists will be able to generate genome-wide libraries containing fluorescently-tagged proteins...

  26. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...strains on the blog, and also recommend this handy guide from OpenWetWare for a more extensive listing of...often ambiguous and should be viewed more as a guideline, since they are typically based on the copy number...

  27. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...plasmids tend to be high copy number. Here, we will consider the replication mechanism of plasmids under stringent...plasmid to balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled...

  28. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...orange and red fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns...bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Reports 7, 42398. PubMed PMID...

  29. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...expression. Take a look at all of the options and decide what might work best for your experiments! This...Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA...

  30. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...channel for more helpful animations, useful protocol videos, and quick tips for lab success.  ...

  31. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for...

  33. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ...Antibody, Addgene gave to me…  An online Antibody Guide!    🎶On the fourth day of Antibody, Addgene gave...

  34. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...decision between some meaningful names…. Watch the video to find out the final four! (Note: For cases where...

  37. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured over the...

  39. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...the 2nd day of CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with...

  40. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of CRISPR, Addgene gave to...

  42. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...activities.  Here, we demonstrate the new system in a video filmed at Addgene:     If you're working in fly...

  44. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...international institution. Requesters will need to provide a brief description of the applicable research ...

  46. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...their paper uncovering ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation...

  47. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...implemented at Addgene. The kitchen and office areas provide multiple streams for composting and recycling in...

  48. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...Blog Possible guest topics Some topics you might consider are: An overview of software tools useful to ...

  50. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...all sequences. However, prime editing and the identification of other Cas proteins continue to expand the...
Showing: 801 - 850 of 888 results