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We narrowed to 574 results for: BASE;

Showing: 321 - 340 of 574 results

  1. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...consistent development and improvement of CRISPR-based technologies over the past decade. CRISPR screens... fact supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large...

  3. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity Purity of...Schematic of the barcode matching system and PCR-based confirmation of transfer plasmids used in Addgene...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in this blog..."The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression...

  5. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...1990s, researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus...actively dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, ...

  6. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...conformational changes, cleavage activity using FRET-based biosensors, and interactions between DNA and proteins...limited to a single measurement. This method is based on the fact that the donor is quenched when FRET...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target genes...Promoter specificity Aside from choosing a promoter based on type of RNA transcript, you will also need to...

  9. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...Tool for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo...

  12. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial antibiotic...commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include information about...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... PAM requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities...toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo ...acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells...

  14. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...cells. A major bottleneck, however, to these IP-based assays is to find (or generate in-house) a pre-validated...develop a hassle-free, highly efficient Cas9 protein-based method for epitope tagging in mammalian stem cells...will have to design your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA...

  17. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...to understand. Classes are based on the main Cas effector; types are based on main function and composition...composition; subtypes and variants are typically based on species of origin. There are currently two classes...

  18. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single origin of replication...into a “pseudoknot” held together by intramolecular base pairs for the protein to be translated. An antisense...

  19. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton...immunogenicity typically associated with viral vector-based therapies. REVeRT supports advanced gene expression...negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that administering...

  20. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...a quick chat can be rather tricky (think people based in different countries). Here social media is a ...References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and job satisfaction...
Showing: 321 - 340 of 574 results