We narrowed to 614 results for: CaS

...worry about vector replication at all. In these cases, transient transfection may be sufficient for your...transfection experiments. Reporters Finally, in some cases a researcher may choose not impose selection on ...organism your experiment takes place in. In many cases, you won’t need to worry about the expression of...

...Alongside there is a second-line team that takes on cases requiring in-depth analysis, data review, in-house...knowledge and judgement. For particularly tricky cases, team discussions can often lead to otherwise unobtainable...patient, attentive, empathetic, and tactful. Occasionally it can also be necessary to take the lead and...

...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method..., relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish ..., and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these...

...approach known as multimodal therapy. Sadly, in many cases the cancer is diagnosed so late that these treatments...success in treating mesothelioma, such as in the case of Paul Zygielbaum. A different approach to immunotherapy...and used throughout the world. New mesothelioma cases could be greatly decreased through a ban on asbestos...

...their data months or years before others. In some cases, typically consortia involving genomic screens, ...the originating researchers, even if they are occasionally beaten to press, as there is so much analysis...track the motion of multiple animals on different occasions via video, generating huge amounts of data. When...

... an intact sample, a cell in the case of ICC or a tissue in the case of IHC. IF is used specifically when...interest within an intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based...

...complement each other to restore the full-expression cassette. AAV’s natural ability to reassemble fragmented...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site...2017) CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756...

...in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently expressed using either...promoters with no tissue specificity, so in some cases they can lead to toxicity and off target effects... an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let'...

...(Mouse IgG2a) Goat anti-mouse IgG2a CASPR anti-CASPR mAb K65/35 (Mouse IgG1) Goat anti-mouse...Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1 (cyan). (B) Cerebellum labeled...

...plasmid with repeating sequences. In some specific cases, multimerization has been observed to occur more...reduce the frequency of multimerization, and in some cases completely prevent the formation of plasmid multimers...Sharratt, 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one...

...environmentally friendly organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based... a little bit about everything or in Jefferson’s case, even a lot about everything. Fast forward a few...received 104 million results (Here’s the top one, in case you’re interested). “But I’m an introvert!” is not...

...can alter the structure of the protein. In some cases this can lead to changes or a complete loss of protein...lipids including those of the cell membrane. In some cases, this can mask epitopes such that immune complexes...only enter cells with disrupted membranes as is the case with dead or dying cells. DNA binding dyes are not...

...In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...

..., and bidirectional transport for AAV1, -8, -9 (Castle et al., 2014). Differences between AAV serotypes...space along an axon, this does not seem to be the case for either AAV1 or AAV9. It should be noted that...PMID: 25555356. PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur,...

...of CRISPR, Addgene gave to me: RNA editing with Cas13.  On the tenth day of CRISPR, Addgene gave to me...to me: methods for adenoviral delivery of CRISPR/Cas9.  On the twelfth day of CRISPR, Addgene gave to ...

...unsuccessful. Listen to the interview with Crystal Shih Podcast breakdown Intro Chapter 1: Who is Crystal Shih?...the audio engineer and producer for the Addgene Podcast.      Additional Resources from Addgene Find more...

...Community.” This post leads readers to our latest podcast all about graduate student life and how establishing...Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business of...

...and a project. Listen to the interview here! Podcast Breakdown (Time : Topic) 0:00 - 0:28 : Niroshi ...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...contains and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid...pBACKBONE-hGene Tip: Often researchers will add a lower case letter to the beginning of their insert abbreviation...