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We narrowed to 599 results for: ELP

Showing: 321 - 340 of 599 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining which ...

  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... your gel. At their most basic, loading controls help you rule out errors like uneven pipetting or irregular...necessary to demonstrate a POI’s absence. It also helps confirm that lanes are loaded evenly. Created with...

  4. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...publication, improving your general writing skills will help you in your academic publishing goals and make you...reach out to your peers and other mentors who can help you. You Can Talk to Your Editors I wish I had...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression... CRISPR has made targeting much easier! With the help of guide RNAs, fusions between Cas proteins and ...

  6. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...world where so much is out of your hands, it’s helpful to focus on something controllable, like experiments...results of a single mouse. Biological replicates help you to determine which responses arise from drug...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...options to consider, and the following factors will help you determine which one(s) are candidates for your...are a number of optogenetic tools now available to help light your path! Resources and references Recommended...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...You’ll need to consider if such properties could help, or harm, your experimental goals when designing... other posts in our Viral Vectors 101 series can help guide you with these, so keep reading! Good luck...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...deposit your piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot...nucleases. Mathew applies his specialized knowledge to help researchers identify the right technologies and ...

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...likely won’t work in a given application is just as helpful as knowing when it will. And remember, antibodies...others in the community can benefit and we can all help improve antibody reproducibility!     References...

  11. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...polybrene (protocol) or DEAE dextran (protocol), which help the viral particle bind to your target cells. If...packaging cell lines to avoid recombination leading to helper virus production." Molecular and Cellular Biology...

  12. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...If this sounds like you, then flow cytometry can help you overcome these challenges and make these types...except one. The fluorescence-minus one panel will help you set up gating during data analysis.  We hope...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...what we discuss. If you can think it, MoClo might help you achieve it! MoClo applications and specialized...2017). If you can dream up an experiment, MoClo can help you achieve it! More recently, a CRISPR MoClo kit...

  16. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...So, labeling a HaloTag or SNAP-tag construct can help you better detect dim structures or low levels of...are comfortable with it or can find a colleague to help you, synthesizing your own ligands can open up more...

  18. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...in healthy and apoptotic cells (9). PA-FPs have helped to revolutionize the field of super resolution ...world of super resolution microscopy, PA-FPs have helped create several new imaging methods, including photoactivation-localization...

  19. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use mammalian plasmids to ...nickases to generate a knockout or edit. This can help reduce off-target activity. Deleting a large region...

  20. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ... to have high expression, so adding this G could help with expression, though it is possible for the plasmid... form of crRNA is expressed and a tracrRNA (the helper RNA that facilitate the processing of the crRNA...
Showing: 321 - 340 of 599 results