We narrowed to 361 results for: ERG

1. The Challenges of Cell Culture

   Type

   Blog Post

   Published

   May 5, 2016, 2:30 p.m.

   ... of tracking how many passages the cells have undergone and when to throw them away and thaw a new batch...

2. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence, the resting phase...

3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...the resident proteins within these compartments undergo significant post-translational modifications including...

4. Plasmids 101: Control Plasmids

   Type

   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...

5. Data Freedom: The Expansion of Data Sharing in Research Publications

   Type

   Blog Post

   Published

   Aug. 5, 2014, 6:51 p.m.

   ...likely won’t be long before other data repositories emerge that allow efficient searching and extraction of...

6. Plasmids 101: The Promoter Region – Let's Go!

   Type

   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ..., 10 General expression mRNA Yeast adjacent, divergently transcribed promoters Inducible with galactose...

7. Plasmids 101: Biotinylation

   Type

   Blog Post

   Published

   Nov. 15, 2018, 1:50 p.m.

   ...gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the general process...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ... A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions through...

9. Five Popular Model Organisms, Part 2

   Type

   Blog Post

   Published

   Sept. 29, 2020, 1:15 p.m.

   ...blog post to see even more genetic systems for emerging model organisms!) Escherichia coli One of the ...

10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    .... https://doi.org/10.1007/978-1-60761-657-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D.,...

11. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...-SspB heterodimerizes under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane...

12. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ...protein. This is clearly the case when proteins undergo post-translational modification with fatty acyl...

13. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...than using more complex protective methods or detergents like CHAPSO, while requiring a lower concentration...

14. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...21561674. PubMed Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient...

15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    Published

    Oct. 11, 2016, 2:30 p.m.

    ...delivered and expressed transiently using a standard detergent, Polyethylene Glycol (PEG), although the application...

16. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    Published

    Aug. 23, 2016, 2:30 p.m.

    ...about the problems you are addressing by using an energetic tone of voice. Your goal is to be conversational...

17. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...site, digestion with the appropriate enzyme will merge two smaller bands visible in a digest of the unmodified...

18. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...at Harvard University currently studying the serotonergic system and behavior.     References 1. Southern...

19. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ...before hand, you’re saving the institute time and energy that would typically be wasted going through some...

20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...-1.0 expresses RFP when the construct has not undergone recombination. This default state was disproportionately...