We narrowed to 8 results for: ERG
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TypeProtocol... lower the pipette tip into the liquid. Do not submerge the pipette itself into the liquid and avoid touching...contaminating the contents. Once the end of the tip is submerged, slowly release the plunger, allowing it to go...
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Pouring LB Agar Plates
TypeProtocol... a water bath at 60 ℃ with sufficient water to submerge ~75% of the bottle containing your molten gel ...removing anything from the autoclave. Partially submerge your molten gel-mix in the 60 ℃ water bath. Pro-Tip... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab. For more information, visit Addgene’s Mammalian... pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For more information, visit Addgene’s Mammalian... -
Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs
TypeProtocol...bare hand to open doors. For those with latex allergies, be aware that some gloves may contain latex. ... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...cells to completely die. After that, the cells may undergo additional selection while the population expands... -
Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...Pro-Tip Because the polyclonal cell pool is always undergoing selection, it’s best to generate monoclonal cell... -
AAV Production in HEK293 Cells
TypeProtocol...cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the cells twice a week during... -
Lentivirus ddPCR Titration
TypeProtocol...Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...