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We narrowed to 361 results for: ERG

Showing: 1 - 20 of 361 results

  1. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...viral service items we employ dozens of pieces of energy-intensive lab equipment on a daily basis. By the...allows a community like Watertown to bulk purchase energy from a higher percentage of renewable sources. ... drawing 100% of our electricity from renewable energy projects right here in New England- the majority...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical... or substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will... will allow us to maintain these particularly energy-intensive systems in a more environmentally responsible...

  2. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...scientists I’ve met began doing research in their undergraduate years. We each have a unique “origin story,”... provide some perspective and advice to any undergraduate interested in getting a taste of the role of...Professor Sometimes teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc...choosing one as their official thesis lab Undergraduate student Roles and responsibilities can vary...would also highly recommend talking to other undergraduate researchers, as well as graduate students (your...’s something you want to dedicate your time and energy to. How can I make the most of my experience in...valuable for anyone in the lab though, including undergrads. I found a lot of career resources on Science...

  3. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with... what is now commonly termed a Course-based Undergraduate Research Experience (CURE). Having incorporated...Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze their ...original research experiences for thousands of undergraduates. The MCC develops and makes freely available...production.   CUREs are beginning to transform undergraduate education in the sciences by making a greater... State College in New Hampshire. He mentors undergraduates conducting NIH- and NSF-funded research on ...Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences can make scientific research...

  4. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    Published
    Sept. 5, 2018, 12:01 p.m.
    ...Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an open-access journal that enables...Addgene. Listen to the interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter ... Springel, Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter 2 - Getting high school...

  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...antibodies. Detergents  Another common family of permeabilization reagents are detergents, such as Triton...these detergents avoid some of the negative effects caused by organic solvents. However, detergents can ...for what reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic...Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the... X-100 and Tween-20 are examples of non-ionic detergents that interact with lipids and proteins in membranes...protein. B) Permeabilizing with the selective detergent, saponin, keeps membrane-associated proteins in... epitopes. C) Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated...

  6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA...with the discovery of CRISPR-transposons by the Sternberg and Zhang labs (Peters et al., 2017; Klompe et...Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated Transposase...the CRISPR-transposon systems developed by the Sternberg lab derives from Vibrio cholerae (V. cholerae ...CRISPR-transposons for bacterial genomes engineering In the Sternberg group’s initial report of VchINT, three separate...Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical Center. He ....     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA...

  7. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction between...fluorophore (the donor) is excited, it can transfer energy directly via resonance to the second fluorophore...fluorophore labeled and that these fluorophores can undergo FRET when they are near each other. How do you ...still develop a successful FRET-based assay since energy transfer only occurs when donor and acceptor are...acceptors >100 Å away from the donor doesn’t result in energy transfer and thus, your specific FRET signal is...donors never “see” FRET acceptors, and thus never undergo FRET. If this nonspecific labeling far exceeds ...experiments will define the maximum and minimum energy transfer values you can expect, thereby establishing...

  8. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...have acquired divergent functions due to mutations accumulated over time? Undergraduates can help to answer...providing authentic research experiences to undergraduate students (1, 2), but educators face significant...Time project provides a framework for these undergraduate research questions. The idea is straightforward...cerevisiae. The two yeasts are thought to have diverged from a common ancestor ~350 million years ago.... have been largely conserved since the species diverged. Tips on using the Pathways Over Time plasmids... in strategies to incorporate research into undergraduate courses and in communicating science to the ...Advancement of Science. (2011). Vision and Change in Undergraduate Biology Education: A Call to Action, Washington...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described by Theodor... the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation and emission...fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted photons) from the excited donor...fluorophore excitation by absorption of a photon 2. Energy transfer from the excited donor directly to the...spectrum of the acceptor fluorophore. Otherwise the energy transferred from the donor will not be able to ...of each other and be oriented appropriately for energy transfer via dipole-dipole interaction. The efficiency...M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission in living...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...the photon's energy is lost, so the energy of an emitted photon is lower than the energy of the absorbed...wavelengths are higher in energy, while photons of higher wavelengths are lower in energy. As such, wavelengths...they promote electrons within atoms to a higher energy state. This process is called excitation. After...a short period, the electrons revert to a lower energy state, whereby a photon is emitted. This step is... due to a process called Fluorescence Resonance Energy Transfer (FRET).   Figure 1: Emission spectra... relationship between a photon's wavelength and energy, how photons can be absorbed and emitted by matter...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., 2017 and 2018). While the Doudna lab ...not been approved for diagnostics yet, but is undergoing validation studies.     Jennifer Tsang contributed...updating this article. References Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT...439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy...442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins...10.1126/science.aaq0179 Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner...Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann...

  12. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...other reagents in biology. Luk H. Vandenberghe, PhD Luk Vandenberghe’s first lab experience was in a virology... Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re excited to grow the team and bring on...worked on vaccines throughout that time. Now Vandenberghe is an Associate Professor at Mass Eye and Ear... to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions with...ultimately make innovation and science slow,” Vandenberghe says. “The continuous creativity that Addgene...Karen Guerin for the journal Molecular Therapy. Vandenberge is also the Grousbeck Family Chair in Gene Therapy...

  13. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Plasmids: History & Biology The Joshua Lederberg Papers Joshua Lederberg's Personal Perspective: Plasmid (1952...Pubmed PMID: 13003535. Lederberg, Joshua, Luigi L. Cavalli, and Esther M. Lederberg. “Sex Compatibility ...did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these...the Fertility, or F-factor discovered by Ester Lederberg in 1952 was noted to integrate into the E. coli...PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    Interesting...well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique which allows the detection ..."a distance-dependent physical process by which energy is transferred non-radiatively from an excited ... may be higher than the signal generated by the energy transfer. On the contrary, if you choose a pair... development of new fluorescent pairs and the emergence of faster measurement systems, this technique .... Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell...

  15. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...CRISPR-transposon system before another exciting new advance emerges! The programmability and targeting power of CRISPR...the directed evolution expertise of the Samuel Sternberg and David Liu labs. Evolving CASTs for new contexts...plasmids here! The new horizons of evoCAST In the emerging world of gene therapy, highly tailored treatments... H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial genome engineering using..., S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded CRISPR-Cas systems...L. H., Chen, A. T., Tolar, J., Osborn, M. J., Sternberg, S. H., & Liu, D. R. (2025). Programmable gene...

  16. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...summer internship information sessions to help undergraduate students with their applications. The society...society currently has 1,283 members, who comprise undergraduates, graduate students, postdocs, faculty members...The society encourages interactions between undergraduates and PhD students so that they can help support...society currently has 352 members, ranging from undergrad all the way up to professors. Oxford Biological...at Imperial College London for students in this emerging field. It has gained over 170 members in its first...or biological societies were you a part of in undergrad or grad school? How did these societies help (...

  17. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ..., so it associates with random DNA sequences (Sternberg et al., 2014). The nuclease lobes are inactive...and active conformations (Bravo et al., 2022; Sternberg et al., 2015). The correct positioning of the ...before cleavage can occur (Jiang & Doudna, 2017; Sternberg et al., 2015).     Figure 8: Comparison...cellular recycling factors to release its handiwork (Sternberg et al., 2014). New discoveries on this topic are...and Computational Biology, National Institute of Allergy and Infectious Diseases. Resources and references...9), 839–843. https://doi.org/10.1038/nbt.2673 Sternberg, S. H., LaFrance, B., Kaplan, M., & Doudna, J.... 110–113. https://doi.org/10.1038/nature15544 Sternberg, S. H., Redding, S., Jinek, M., Greene, E. C.,...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. In the Lindberg Lab we ...blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. Iris Lindberg is a professor...PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in PubMed). While we found that nearly ...neural and endocrine cells. She can be emailed at ilindberg@som.umaryland.edu.   Additional Resources on the...

  19. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ... any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his microbiology lab course...wear a mask. (@GermJuggler) February 13, 2021 Westenberg, a Professor of Biological Sciences at Missouri...by using a thicker bacterial suspension,” says Westenberg. In addition to streaking for single colonies...them into different strains of E. coli,” says Westenberg. During the transformation experiment, he plans...efficiency.  Aside from the Streak Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing...

  20. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...fluorescent microscopy. Collimated (non-converging or diverging) excitation light exits the microscope ...its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique...GFP. Because red light has approximately half the energy of blue light, two photons of red light are needed...Although the excitation light is completely reflected, energy is propagated into the sample via an evanescent...the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes that project ultra-thin...
Showing: 1 - 20 of 361 results