We narrowed to 51 results for: ERG

...-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer...

...CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862...plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214857...) Expression of ChR2(H134R)-EYFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 213947...GCaMP6f AAV construct expressing GCaMP6f driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_... mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer Gordon Fishell James...

...cleavage. Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding...stability PEmax — optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced editing... ssisted T argeting), developed by Samuel H. Sternberg’s lab . Many of these systems are based on naturally...argeting E lements), developed by the Abudayyeh - Gootenberg lab , also builds off prime editing. PASTE uses...Welch, M. M., Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J.... S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., Oura, S., Holmes, B., Tanaka...Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O.,...

...Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons...fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons...Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion...conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS...10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable...to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts...neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons Lentiviral Human Direct Conversion...

... Fluorescence) Resonance Energy Transfer (FRET) is a process by which energy is non-radiatively transferred...resonance energy transfer mCerulean, mVenus, and Amber (mVenus Y67C) constructs for measuring energy transfer...microscopy (FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor lifetime...et al. (2006) Quantitative understanding of the energy transfer between fluorescent proteins connected...acceptors Koushik et al. (2009) Anomalous surplus energy transfer observed with multiple FRET acceptors ... protein acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid...

...and Andrew Scharenberg 22502 pCEP4-tat N/A Expression of tat . Increases viral titer. Sergey Kasparov 22500...See plasmid 24150 for hygromycin selection. Bob Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven...pSLCAR-CD19-28z 3rd Modular CD28-CD3z CAR backbone. See Bloemberg et al. (2020) for additional CAR backbones. Scott... 2nd and 3rd generation transfer plasmids. Bob Weinberg 12251 pMDLg/pRRE 3rd Contains gag and pol . Can...22500 pNHP 2nd Contains gag , pol , rev , and tat . Sergey Kasparov Return to top Popular Lentiviral Envelope...pCMV-VSV-G VSV-G-expressing envelope plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid...22501 pHEF-VSVG VSV-G-expressing envelope plasmid. Sergey Kasparov Return to top Additional Resources Lentiviral...

...Targeted sensors for glutamatergic neurotransmission Targeted sensors for glutamatergic neurotransmission...fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET) efficiency of a pair of fluorescent...Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics...Fluorescent sensor of ATP:ADP ratio (PercevalHR) Imaging energy status in live cells with a fluorescent biosensor...and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of... acetylcholine sensor for monitoring in vivo cholinergic activity (GACh3.0) (Constitutive or Cre-dependent... acetylcholine sensor for monitoring in vivo cholinergic activity. Nat Methods. 2020 Sep 28. pii: 10.1038...

...immortalized cells. See the Weinberg Lab page for additional resistance variants. Bob Weinberg 1773 pBABE-hygro-..., and plasmid 1774 for neomycin selection. Bob Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA...gag and pol . Use with an envelope plasmid. Bob Weinberg 12371 pCL-Eco Contains MoMLV gag , pol , and env...envelope protein. Use with packaging plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid...22501 pHEF-VSVG VSV-G-expressing envelope plasmid. Sergey Kasparov Return to top Additional Resources Gamma-Retroviral...

... CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson 83441 pSOD1E133insTT-AcGFP1...TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV ALS Shawn Ferguson 133755 pLVX-Puro-...TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP CMV ALS Shawn Ferguson 133757 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP CMV ALS Shawn Ferguson 133759 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP CMV ALS Shawn Ferguson 133761 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43...U6 ALS Shawn Ferguson 221553 pPB-EF1A-Puro-hTBK1-EGFP TBK1 GFP EF1-alpha ALS Shawn Ferguson 221554 pPB-...

...watermelon mutant clones for your course-based undergraduate research experience (CURE) projects....Community (MCC) Collection Blog post: Course-Based Undergraduate Research Experiences (CUREs) (Link opens in ...window) develops protein-centric Course-based Undergraduate Research Experiences (CUREs) centered on a single...opens in a new window) Length of course-based undergraduate research experiences (CURE) impacts student ...Increasing access for biochemistry research in undergraduate education: The malate dehydrogenase CURE community...

...synthesis, and increases energy production through glycolysis to fuel these energy-intensive processes. mTORC1...signals, including amino acids, stress, oxygen, energy levels, and growth factors. mTORC1 integrates these...including an abundant supply of macromolecules and energy, as well as the presence of both pro-proliferative...machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov...

...minBG-SYFP2-WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA...minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2...minBG-SYFP2-WPRE3-BGHpA AiP13038 AiE0743m_3xC2 SYFP2 Cholinergic interneurons Striatum 214848 pAAV-AiE0743m_3xC2...minBG-iCre(R297T)-BGHpA AiP13780 AiE0743m_3xC2 Cre Cholinergic interneurons Striatum 214858 pAAV-AiE0873m_3xC2...10aa-H2B-WPRE3-BGHpA AiP14496 AiE0873m_3xC2 SYFP2 Cholinergic interneurons Striatum 214868 pAAV-AiE0873m_3xC2...minBG-iCre(R297T)-BGHpA AiP15138 AiE0873m_3xC2 Cre Cholinergic interneurons Striatum 191706 pAAV-AiE0779m_3xC2...

...Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. 2017. Enhanced...PMID: 27984730 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...Science . 343(6166):80-4. PMID: 24336569 Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost...

...activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein... editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ...J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature . ...aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

...production, all antibody preps are titered and undergo stringent quality control by Addgene or a trusted...Buffer After affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The...distributed. Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs...

... BRET energy donor and HaloTag protein, labeled with the NanoBRET 618 fluorophore, as the energy acceptor...Assays NanoBRET (Link opens in a new window) is an energy transfer technique designed to measure molecular...

...indicators . (Aggarwal et al. bioRxiv. 2025) Monitor dopaminergic activity in vivo with improved green and red... Acad Sci USA. 2017) Reprogram astrocytes to dopaminergic neurons using three transcription factors in...resource was created with support from the Chan Zuckerberg Initiative (Link opens in a new window) ....

...delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786. PubMed (...Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range..., Fuzik J, Fürth D, Fenno LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A...

.... Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase... NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as...

...visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA...NgoMIV, AgeI, SpeI, PstI For more info, visit iGEM: Freiberg Standard (RFC 25) Back to Top...