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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 556 results for: ESC;

Showing: 321 - 340 of 556 results

  2. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular...

  6. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...They found that the cataracts phenotype could be rescued by zygote co-injection of Cas9 and a single-guide...

  7. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd... deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and...

  10. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...for binding to the target protein. The protocol describes going through two rounds of phage display to increase...interest. The Seeger lab used the ELISA technique described in their protocol because it is less susceptible...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...visualize proteins in vivo by tagging them with fluorescent proteins to study localization or purify proteins...Expression Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant...

  12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...

  13. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential decay process we observed, while...model for each region using stochastic gradient descent in Pytorch, which gave us the maximum likelihood...

  14. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...revealed that cell lines from individuals of African descent have approximately 20% more single nucleotide polymorphisms...are more common in individuals of African descent.Correcting the bias  While their thorough analysis uncovered...

  15. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

  16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...targeted approach using RNPs has recently been described in vitro, with the hope of eventually using this...is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets...

  17. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...variations, including many that use auxotrophic rescue genes as reporters and some that repress instead...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...

  18. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...quantitative reporters and use a substrate to produce bioluminescence. Luciferases usually have shorter mRNA and ...tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways...

  20. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...
Showing: 321 - 340 of 556 results